Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast.
Meiotic cohesins modulate chromosome compaction during meiotic prophase in fission yeast.
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DOI：
10.1083/jcb.200605074
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发表时间：
2006-08-14
期刊：
影响因子：
--
通讯作者：
Hiraoka Y
中科院分区：
文献类型：
--
作者：
Ding DQ;Sakurai N;Katou Y;Itoh T;Shirahige K;Haraguchi T;Hiraoka Y
减数分裂粘连蛋白 Rec8 是两轮减数分裂过程中染色体逐步分离所必需的。通过直接测量裂殖酵母裂殖酵母活细胞中的染色体压缩，我们发现减数分裂粘连蛋白在减数分裂前期染色体压缩中的另一个作用。在没有 Rec8 的情况下，染色体相对于野生型细胞来说是解压缩的。相反，粘连蛋白相关蛋白 Pds5 的缺失会导致过度致密。尽管这种超压缩需要Rec8，但在没有Pds5的情况下，Rec8与染色质的结合会减少，表明Pds5促进Rec8的染色体关联。为了解释这些观察结果，我们提出减数分裂前期染色体被组织为从包含 Rec8 的轴发出的染色质环：Rec8 的缺失会破坏轴，导致染色体杂乱，而 Rec8 负载的减少会导致纵向压缩的轴，具有更少的附着点和更长的染色质环。
The meiotic cohesin Rec8 is required for the stepwise segregation of chromosomes during the two rounds of meiotic division. By directly measuring chromosome compaction in living cells of the fission yeast Schizosaccharomyces pombe, we found an additional role for the meiotic cohesin in the compaction of chromosomes during meiotic prophase. In the absence of Rec8, chromosomes were decompacted relative to those of wild-type cells. Conversely, loss of the cohesin-associated protein Pds5 resulted in hypercompaction. Although this hypercompaction requires Rec8, binding of Rec8 to chromatin was reduced in the absence of Pds5, indicating that Pds5 promotes chromosome association of Rec8. To explain these observations, we propose that meiotic prophase chromosomes are organized as chromatin loops emanating from a Rec8-containing axis: the absence of Rec8 disrupts the axis, resulting in disorganized chromosomes, whereas reduced Rec8 loading results in a longitudinally compacted axis with fewer attachment points and longer chromatin loops.
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影响因子：
2.1
作者：
Chikashige, Y;Kurokawa, R;Hiraoka, Y
通讯作者：
Hiraoka, Y
DOI：
10.1073/pnas.93.7.2795
发表时间：
1996-04-02
影响因子：
11.1
作者：
Heng, HHQ;Chamberlain, JW;Moens, PB
通讯作者：
Moens, PB
影响因子：
64.8
作者：
Lengronne, A;Katou, Y;Mori, S;Yokobayashi, S;Kelly, GP;Itoh, T;Watanabe, Y;Shirahige, K;Uhlmann, F
通讯作者：
Uhlmann, F
影响因子：
56.9
作者：
CHIKASHIGE, Y;DING, DQ;HIRAOKA, Y
通讯作者：
HIRAOKA, Y
影响因子：
7.8
作者：
Bahler, J;Wyler, T;Loidl, J;Kohli, J
通讯作者：
Kohli, J