Promoting RNA helical stacking via A-minor junctions.
Promoting RNA helical stacking via A-minor junctions.
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DOI：
10.1093/nar/gkq748
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发表时间：
2011-02
影响因子：
14.9
通讯作者：
Jaeger L
中科院分区：
文献类型：
--
作者：
Geary C;Chworos A;Jaeger L
RNA分子利用普遍的结构基序折叠和组装成明确的3D结构。a -小结是一类RNA基序，在天然RNA中特异性地控制螺旋的同轴堆叠。利用一种灵敏的自组装超分子系统，通过天然聚丙烯酰胺凝胶电泳和原子力显微镜比较了几种天然的和以前未发现的A-minor连接。这类模基遵循一个拓扑规则，可以容纳各种可互换的a小调相互作用与不同的局部结构基。总的来说，根据其功能自组装行为，可以区分两种不同类型的A-minor连接：一组利用triloops或GNRA和GNRA样环与螺旋组装，而另一组利用更复杂的环特异性三级受体获得更高的稳定性。这项研究证明了RNA的不同结构基序如何有助于形成拓扑等效的螺旋堆栈。它还举例说明了基于它们的三级结构特征而不是二级结构特征对RNA基序进行分类的需要。在纳米生物技术和合成生物学中，A-minor结规则可用于RNA的三级结构预测和RNA部分的合理设计。
RNA molecules take advantage of prevalent structural motifs to fold and assemble into well-defined 3D architectures. The A-minor junction is a class of RNA motifs that specifically controls coaxial stacking of helices in natural RNAs. A sensitive self-assembling supra-molecular system was used as an assay to compare several natural and previously unidentified A-minor junctions by native polyacrylamide gel electrophoresis and atomic force microscopy. This class of modular motifs follows a topological rule that can accommodate a variety of interchangeable A-minor interactions with distinct local structural motifs. Overall, two different types of A-minor junctions can be distinguished based on their functional self-assembling behavior: one group makes use of triloops or GNRA and GNRA-like loops assembling with helices, while the other takes advantage of more complex tertiary receptors specific for the loop to gain higher stability. This study demonstrates how different structural motifs of RNA can contribute to the formation of topologically equivalent helical stacks. It also exemplifies the need of classifying RNA motifs based on their tertiary structural features rather than secondary structural features. The A-minor junction rule can be used to facilitate tertiary structure prediction of RNAs and rational design of RNA parts for nanobiotechnology and synthetic biology.
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影响因子：
38.3
作者：
通讯作者：
--
影响因子：
56.9
作者：
Chworos, A;Severcan, I;Jaeger, L
通讯作者：
Jaeger, L
影响因子：
5.6
作者：
Abramovitz, DL;Pyle, AM
通讯作者：
Pyle, AM
影响因子：
5.6
作者：
Laing, Christian;Schlick, Tamar
通讯作者：
Schlick, Tamar
影响因子：
14.9
作者：
Geary C;Baudrey S;Jaeger L
通讯作者：
Jaeger L