Different modes of state transitions determine pattern in the Phosphatidylinositide-Actin system.
Different modes of state transitions determine pattern in the Phosphatidylinositide-Actin system.
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DOI：
10.1186/1471-2121-12-42
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发表时间：
2011-10-07
期刊：
影响因子：
--
通讯作者：
Schroth-Diez B
中科院分区：
文献类型：
--
作者：
Gerisch G;Ecke M;Wischnewski D;Schroth-Diez B
在运动的极化细胞中，肌动蛋白系统发生分化，允许前端突出，尾部缩回。这种分化与质膜中的磷酸肌醇模式有关。在这里研究的高活力盘基网柄菌细胞中，前端由产生 PI(3,4,5) 三磷酸 (PIP3) 的 PI3 激酶主导，尾部由 PI3-磷酸酶 PTEN 主导，将 PIP3 水解为 PI(4,5) 二磷酸。为了研究从头细胞极化，我们首先解聚肌动蛋白，随后记录与 PTEN 相关的肌动蛋白模式的自发重组。在解聚恢复的短暂阶段，对称肌动蛋白模式与不对称肌动蛋白模式周期性交替。向不对称性的转变与 PTEN 的单侧膜结合一致。肌动蛋白和 PTEN 系统的状态转换模式不同。肌动蛋白系统中的转变以波的形式传播，这些波通过自发波动的放大在单个位点引发。在 PTEN 缺失的细胞中，这些波仍然以正常速度传播，但失去了规律的周期性。 PTEN 的膜结合是在富含 PTEN 的连贯区域的边界处以扩展和回归梯度的形式诱导的。肌动蛋白组织中的状态转变和从细胞质到膜结合 PTEN 的可逆转变是同步的，但它们的模式不同。肌动蛋白组织的转变独立于 PTEN，但当 PTEN 存在时，它们与这种 PIP3 降解磷酸酶的膜结合的周期性变化相耦合。 PTEN 振荡与趋化细胞的运动模式有关。
In a motile polarized cell the actin system is differentiated to allow protrusion at the front and retraction at the tail. This differentiation is linked to the phosphoinositide pattern in the plasma membrane. In the highly motile Dictyostelium cells studied here, the front is dominated by PI3-kinases producing PI(3,4,5)tris-phosphate (PIP3), the tail by the PI3-phosphatase PTEN that hydrolyses PIP3 to PI(4,5)bis-phosphate. To study de-novo cell polarization, we first depolymerized actin and subsequently recorded the spontaneous reorganization of actin patterns in relation to PTEN. In a transient stage of recovery from depolymerization, symmetric actin patterns alternate periodically with asymmetric ones. The switches to asymmetry coincide with the unilateral membrane-binding of PTEN. The modes of state transitions in the actin and PTEN systems differ. Transitions in the actin system propagate as waves that are initiated at single sites by the amplification of spontaneous fluctuations. In PTEN-null cells, these waves still propagate with normal speed but loose their regular periodicity. Membrane-binding of PTEN is induced at the border of a coherent PTEN-rich area in the form of expanding and regressing gradients. The state transitions in actin organization and the reversible transition from cytoplasmic to membrane-bound PTEN are synchronized but their patterns differ. The transitions in actin organization are independent of PTEN, but when PTEN is present, they are coupled to periodic changes in the membrane-binding of this PIP3-degrading phosphatase. The PTEN oscillations are related to motility patterns of chemotaxing cells.
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影响因子：
8.6
作者：
Gamba, A.;Kolokolov, I.;Ortenzi, G.
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作者：
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