Profiling and characterization of miRNAs associated with intramuscular fat content in Yorkshire pigs
Profiling and characterization of miRNAs associated with intramuscular fat content in Yorkshire pigs
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约克夏猪肌内脂肪含量相关 miRNA 的分析和表征
DOI：
10.1080/10495398.2019.1573191
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发表时间：
2020-05
影响因子：
3.7
通讯作者：
J.H. Cao
中科院分区：
文献类型：
--
作者：
Y. Sun;H.Y. Liu;S. Wang;R.M. Ren;Q. Dong;J.H. Xie;J.H. Cao
mirna是一种短的非编码rna，通过结合靶mRNA的互补区域来转录后调节基因表达。与猪肌内脂肪沉积（IMF）含量相关的mirna仍有待研究，这是一项重要的肉质性状。本研究采集了234只体重为90 kg的约克猪背最长肌（ldm），采用高（2.94±0.04%）和低（1.62±0.02%）IMF样本各5只的双尾组进行miRNA深度测序。结果表明，共鉴定出268个成熟mirna，其中70个是已知的，162个在物种中保守，36个在猪中特异性鉴定。此外，28个参与脂肪形成的mirna在两组中差异表达，其中5个mirna使用茎环引物进行了定量PCR （qPCR）验证。我们的结果可以作为理解miRNA在猪的IMF发展中的作用的基础。本研究鉴定的mirna可用于猪种群IMF性状的研究，并将为肉质调控机制的研究提供进一步的线索。
Abstract miRNAs are short noncoding RNAs that post-transcriptionally regulate gene expression by binding to complementary regions of the target mRNA. The miRNAs associated with the deposition of intramuscular fat (IMF) content in pigs, which is an important meat quality trait, still remain to be investigated. In this study, the longissimus dorsi muscles (LDMs) from 234 individuals were collected from Yorkshire pigs at 90 kg body weight and the miRNA deep sequencing was conducted by using two tailed groups which were taken five individuals each from high (2.94 ± 0.04%) and low (1.62 ± 0.02%) IMF samples. The results showed that total 268 mature miRNAs were identified, of which 70 were previously known, 162 were conserved among species and 36 were identified specifically in pigs. Moreover, 28 miRNAs involved in adipogenesis were differentially expressed in the two groups, and five out of 16 miRNAs were validated by quantitative PCR (qPCR) using stem loop primers. Our results may serve as a fundamental basis for understanding the roles of miRNA in IMF development in pigs. The miRNAs identified in our study can be utilized for research IMF trait in pig population and will provide further clues to the study of meat quality regulatory mechanisms.
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miR-425-5p 抑制猪肌内前脂肪细胞的分化和增殖
影响因子：
5.6
作者：
Chen FF;Xiong Y;Peng Y;Gao Y;Qin J;Chu GY;Pang WJ;Yang GS
通讯作者：
Yang GS
影响因子：
14.9
作者：
John E;Wienecke-Baldacchino A;Liivrand M;Heinäniemi M;Carlberg C;Sinkkonen L
通讯作者：
Sinkkonen L
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
DOI：
10.1016/s0021-9258(18)55987-1
发表时间：
1951-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
J. Folch;I. Ascoli;M. Lees;J. A. Meath;F. Lebaron
通讯作者：
J. Folch;I. Ascoli;M. Lees;J. A. Meath;F. Lebaron