miR-425-5p Inhibits Differentiation and Proliferation in Porcine Intramuscular Preadipocytes.
miR-425-5p Inhibits Differentiation and Proliferation in Porcine Intramuscular Preadipocytes.
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miR-425-5p 抑制猪肌内前脂肪细胞的分化和增殖
DOI：
10.3390/ijms18102101
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发表时间：
2017-10-06
影响因子：
5.6
通讯作者：
Yang GS
中科院分区：
文献类型：
--
作者：
Chen FF;Xiong Y;Peng Y;Gao Y;Qin J;Chu GY;Pang WJ;Yang GS
肌内脂肪（IMF）含量影响猪肉的嫩度、多汁性和风味。越来越多的研究集中在microRNA（miRs）在猪肌内前脂肪细胞发育过程中的功能。已有研究证明miR-425- 5 p在猪骨骼肌中富集，在多种生理过程中发挥重要作用，但其在肌内脂肪形成过程中的作用尚不清楚。为了探讨miR-425- 5 p在猪肌内脂肪形成中的作用，分别使用miR-425- 5 p的agomir和抑制剂在肌内前脂肪细胞中进行miR-425- 5 p的过表达和敲低。我们的研究结果表明，miR-425- 5 p的agomir显著抑制肌内脂肪形成分化，并下调脂肪形成标记基因PPARγ、FABP 4和FABP 4的表达水平，而其抑制剂促进脂肪形成。有趣的是，阿戈米尔通过下调细胞周期蛋白B和细胞周期蛋白E来抑制猪肌内前脂肪细胞的增殖。此外，我们证明了miR-425- 5 p通过靶向和抑制KLF 13的翻译来抑制脂肪形成。综上所述，我们的研究结果表明，miR-425- 5 p是一种新型的猪肌内脂肪生成抑制剂，可能通过靶向KLF 13，随后下调PPARγ。
Intramuscular fat (IMF) content affects the tenderness, juiciness, and flavor of pork. An increasing number of studies are focusing on the functions of microRNAs (miRs) during porcine intramuscular preadipocyte development. Previous studies have proved that miR-425-5p was enriched in porcine skeletal muscles and played important roles in multiple physiological processes; however, its functions during intramuscular adipogenesis remain unclear. To explore the role of miR-425-5p in porcine intramuscular adipogenesis, miR-425-5p agomir and inhibitor were used to perform miR-425-5p overexpression and knockdown in intramuscular preadipocytes, respectively. Our results showed that the agomir of miR-425-5p dramatically inhibited intramuscular adipogenic differentiation and downregulated the expression levels of adipogenic marker genes PPARγ, FABP4, and FASN, whereas its inhibitor promoted adipogenesis. Interestingly, the agomir repressed proliferation of porcine intramuscular preadipocytes by downregulation of cyclin B and cyclin E. Furthermore, we demonstrated that miR-425-5p inhibited adipogenesis via targeting and repressing the translation of KLF13. Taken together, our findings identified that miR-425-5p is a novel inhibitor of porcine intramuscular adipogenesis possibly through targeting KLF13 and subsequently downregulating PPARγ.
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