Cytoplasmic foci are sites of mRNA decay in human cells.
Cytoplasmic foci are sites of mRNA decay in human cells.
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DOI：
10.1083/jcb.200309008
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发表时间：
2004-04
期刊：
影响因子：
--
通讯作者：
Séraphin B
中科院分区：
文献类型：
--
作者：
Cougot N;Babajko S;Séraphin B
理解基因表达控制需要定义mRNA周转的分子和细胞基础。我们以前已经表明，人类去帽因子hDcp 2和hDcp 1a集中在特定的细胞质结构。在这里，我们发现与5′-3′ mRNA降解相关的hCcr 4、hDcp 1b、hLsm和rck/p54蛋白也定位于这些结构，而参与帽核苷酸降解的DcpS则位于细胞核内。使用荧光共振能量转移的功能分析显示，hDcp 1a和hDcp 2在体内相互作用，在这些结构中，被证明不同于先前描述的应力颗粒。我们的数据表明，这些新的结构是动态的，因为它们消失时，mRNA的破坏被废除的抑制剂治疗。在RNAi介导的Xrn 1核酸外切酶失活后，poly（A）+ RNA在这些结构中的积累表明它们代表活性mRNA衰变位点。在人类细胞的特定亚细胞位置发生5′-3′ mRNA衰变，表明真核细胞的细胞质可能比以前预期的更有组织。
Understanding gene expression control requires defining the molecular and cellular basis of mRNA turnover. We have previously shown that the human decapping factors hDcp2 and hDcp1a are concentrated in specific cytoplasmic structures. Here, we show that hCcr4, hDcp1b, hLsm, and rck/p54 proteins related to 5′–3′ mRNA decay also localize to these structures, whereas DcpS, which is involved in cap nucleotide catabolism, is nuclear. Functional analysis using fluorescence resonance energy transfer revealed that hDcp1a and hDcp2 interact in vivo in these structures that were shown to differ from the previously described stress granules. Our data indicate that these new structures are dynamic, as they disappear when mRNA breakdown is abolished by treatment with inhibitors. Accumulation of poly(A)+ RNA in these structures, after RNAi-mediated inactivation of the Xrn1 exonuclease, demonstrates that they represent active mRNA decay sites. The occurrence of 5′–3′ mRNA decay in specific subcellular locations in human cells suggests that the cytoplasm of eukaryotic cells may be more organized than previously anticipated.
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DOI：
10.1083/jcb.136.4.761
发表时间：
1997-02-24
期刊：
The Journal of cell biology
影响因子：
--
作者：
Bashkirov VI;Scherthan H;Solinger JA;Buerstedde JM;Heyer WD
通讯作者：
Heyer WD
影响因子：
2.1
作者：
Akao, Y;Yoshida, H;Usukura, J
通讯作者：
Usukura, J
影响因子：
14.9
作者：
DAN, L;YUNIS, JJ
通讯作者：
YUNIS, JJ
影响因子：
16
作者：
Förch, P;Puig, O;Valcárcel, J
通讯作者：
Valcárcel, J
影响因子：
10.5
作者：
Allmang, C;Petfalski, E;Mitchell, P
通讯作者：
Mitchell, P