Serotonin Increases Cilia-Driven Particle Transport via an Acetylcholine-Independent Pathway in the Mouse Trachea
Serotonin Increases Cilia-Driven Particle Transport via an Acetylcholine-Independent Pathway in the Mouse Trachea
复制标题
血清素通过小鼠气管中乙酰胆碱独立的途径增加纤毛驱动的颗粒运输
作者：
P. König;B. Krain;G. Krasteva;W. Kummer
背景气道中的粘膜纤毛清除是由纤毛细胞的协调搏动驱动的。经典的神经介质，如去甲肾上腺素和乙酰胆碱增加纤毛搏动频率，从而纤毛驱动的运输。尽管神经介质5-羟色胺在无脊椎动物中具有纤毛刺激作用，并被认为可以从气道上皮释放乙酰胆碱，但其在脊椎动物气道中调节纤毛功能的作用尚未确立。方法/主要发现我们研究了5-羟色胺对纤毛搏动频率和纤毛驱动的颗粒运输在急性切除淹没小鼠气管的影响，并通过免疫组化确定了该组织中5-羟色胺的来源。5-羟色胺（100 µ M）增加纤毛搏动频率（8.9 ± 1.2 Hz至17.0 ± 2.7 Hz）和颗粒转运速度（38.9 ± 4.6 µ m/s至83.4 ± 8.3 µ m/s），其程度与超最大剂量的ATP相当。麦角新碱（100 µ M）完全阻止了颗粒传输速度的增加。阿托品（1 µ M）阻断毒蕈碱受体并不能降低5-羟色胺的作用，尽管它能有效阻止毒蕈碱（100 µ M）介导的颗粒转运速度增加。免疫组化显示肥大细胞中的5-羟色胺指向肥大细胞和血小板作为5-羟色胺的可能内源性来源。结论/意义这些结果表明，5-羟色胺是一种可能的内源性介质，可以增加纤毛驱动的运输独立于乙酰胆碱激活肥大细胞和血小板。
Background Mucociliary clearance in the airways is driven by the coordinated beating of ciliated cells. Classical neuromediators such as noradrenalin and acetylcholine increase ciliary beat frequency and thus cilia-driven transport. Despite the fact that the neuromediator serotonin is ciliostimulatory in invertebrates and has been implied in releasing acetylcholine from the airway epithelium, its role in regulating cilia function in vertebrate airways is not established. Methodology/Principal Findings We examined the effects of serotonin on ciliary beat frequency and cilia-driven particle transport in the acutely excised submerged mouse trachea and determined the sources of serotonin in this tissue by immunohistochemistry. Serotonin (100 µM) increased cilary beat frequency (8.9±1.2 Hz to 17.0±2.7 Hz) and particle transport speed (38.9±4.6 µm/s to 83.4±8.3 µm/s) to an extent that was comparable to a supramaximal dose of ATP. The increase in particle transport speed was totally prevented by methysergide (100 µM). Blockade of muscarinic receptors by atropine (1 µM) did not reduce the effect of serotonin, although it was effective in preventing the increase in particle transport speed mediated by muscarine (100 µM). Immunohistochemistry demonstrated serotonin in mast cells pointing towards mast cells and platelets as possible endogenous sources of serotonin. Conclusions/Significance These results indicate that serotonin is a likely endogenous mediator that can increase cilia-driven transport independent from acetylcholine during activation of mast cells and platelets.
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DOI：
10.1172/jci15217
发表时间：
2002-03
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
M. Knowles;R. Boucher
通讯作者：
M. Knowles;R. Boucher
纤毛气管上皮细胞中的毒蕈碱信号传导：对 Ca2+ 和纤毛跳动的双重影响。
DOI：
10.1152/ajplung.1997.272.2.l301
发表时间：
1997
期刊：
The American journal of physiology.
影响因子：
--
作者：
Salathe,M;Lipson,EJ;Ivonnet,PI;Bookman,RJ
通讯作者：
Bookman,RJ
血小板释放血清素在接触敏感性启动中的作用。
影响因子：
2.8
作者：
Askenase,PW;Geba,GP;Levin,J;Ratzlaff,RE;Anderson,GM;Ushio,H;Ptak,W;Matsuda,H
通讯作者：
Matsuda,H
DOI：
10.1165/rcmb.2008-0102oc
发表时间：
2009-03-01
影响因子：
6.4
作者：
Fernandez-Gonzalez, Angeles;Kourembanas, Stella;Mitsialis, S. Alex
通讯作者：
Mitsialis, S. Alex
贻贝切除鳃中的热通量、氧通量和线粒体氧化还原状态与氧气可用性和纤毛活动的函数关系。
DOI：
10.1002/jez.1402650102
发表时间：
1993
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
Doeller,JE;Kraus,DW;Shick,JM;Gnaiger,E
通讯作者：
Gnaiger,E