In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals.

In vivo bioluminescent imaging (BLI): noninvasive visualization and interrogation of biological processes in living animals.
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DOI:
10.3390/s110100180
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发表时间:
2011
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Ripp S
Ripp S
中科院分区:
其他
文献类型:
--
作者:
Close DM;Xu T;Sayler GS;Ripp S

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体内生物发光成像(BLI)越来越多地被用作现代生物学研究的方法。该过程涉及使用表达生物报告细胞发出的光对活动物进行非侵入性询问,已被应用于研究广泛的生物分子功能,如基因功能,药物发现和开发,细胞运输,蛋白质-蛋白质相互作用,特别是肿瘤发生,癌症治疗和疾病进展。本文将回顾各种生物报告/生物传感器集成的BLI和讨论如何BLI被应用到一个新的视觉理解的生物过程中的活的有机体。
In vivo bioluminescent imaging (BLI) is increasingly being utilized as a method for modern biological research. This process, which involves the noninvasive interrogation of living animals using light emitted from luciferase-expressing bioreporter cells, has been applied to study a wide range of biomolecular functions such as gene function, drug discovery and development, cellular trafficking, protein-protein interactions, and especially tumorigenesis, cancer treatment, and disease progression. This article will review the various bioreporter/biosensor integrations of BLI and discuss how BLI is being applied towards a new visual understanding of biological processes within the living organism.
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