Allele frequencies in the VRN-A1, VRN-B1 and VRN-D1 vernalization response and PPD-B1 and PPD-D1 photoperiod sensitivity genes, and their effects on heading in a diverse set of wheat cultivars (Triticum aestivum L.).
Allele frequencies in the VRN-A1, VRN-B1 and VRN-D1 vernalization response and PPD-B1 and PPD-D1 photoperiod sensitivity genes, and their effects on heading in a diverse set of wheat cultivars (Triticum aestivum L.).
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DOI：
10.1007/s11032-014-0034-2
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Karsai I
中科院分区：
文献类型：
--
作者：
Kiss T;Balla K;Veisz O;Láng L;Bedő Z;Griffiths S;Isaac P;Karsai I
谷物的抽穗是由复杂的遗传和环境因素决定的，其中春化和光敏感基因起决定性作用。我们的目的是利用诊断性分子标记在全球683个小麦基因型中确定VRN-A1、VRN-B1、VRN-D1、PPD-B1和PPD-D1的主要等位基因类型，并研究这些等位基因对田间抽穗的影响。VRN-A1、VRN-B1和VRN-D1等位基因的显性频率较低。57%的品种携带PPD-D1a光周期不敏感等位基因，在亚洲和欧洲品种中最为常见。来自亚洲、美洲和欧洲的22%的基因型携带PPD-B1光周期不敏感等位基因。根据基因拷贝数和拷贝间结构鉴定出9个ppd - b1不敏感等位基因。PPD-D1、PPD-B1和VRN-D1等位基因组成显著影响抽穗，共解释了37.5%的表型变异。当考虑PPD-B1拷贝间结构而不是整体PPD-B1类型（敏感与不敏感）时，基因模型的作用增加到39.1%。作为单一成分，PPD-D1具有最重要的作用（占表型方差的28.0%），其次是PPD-B1（占PPD-B1_overall的12.3%，占PPD-B1_intercopy的15.1%）和VRN-D1（2.2%）。在PPD-B1内的标记等位基因之间以及VRN-D1与两个PPD1基因之间发现了显著的基因相互作用。最早抽穗的基因型为PPD-D1和PPD-B1光周期不敏感等位基因，VRN-D1春季等位基因和VRN-A1、VRN-B1冬季等位基因。这种组合只能在南欧和亚洲的基因型中检测到。无论VRN1基因的等位基因组成如何，迟穗基因型均具有PPD1基因的敏感等位基因。田间条件下，最早和最晚群体的抽穗期相差10天。本文的在线版本（doi:10.1007/s11032-014-0034-2）含有补充资料，仅供授权用户使用。
Heading of cereals is determined by complex genetic and environmental factors in which genes responsible for vernalization and photoperiod sensitivity play a decisive role. Our aim was to use diagnostic molecular markers to determine the main allele types in VRN-A1, VRN-B1, VRN-D1, PPD-B1 and PPD-D1 in a worldwide wheat collection of 683 genotypes and to investigate the effect of these alleles on heading in the field. The dominant VRN-A1, VRN-B1 and VRN-D1 alleles were present at a low frequency. The PPD-D1a photoperiod-insensitive allele was carried by 57 % of the cultivars and was most frequent in Asian and European cultivars. The PPD-B1 photoperiod-insensitive allele was carried by 22 % of the genotypes from Asia, America and Europe. Nine versions of the PPD-B1-insensitive allele were identified based on gene copy number and intercopy structure. The allele compositions in PPD-D1, PPD-B1 and VRN-D1 significantly influenced heading and together explained 37.5 % of the phenotypic variance. The role of gene model increased to 39.1 % when PPD-B1 intercopy structure was taken into account instead of overall PPD-B1 type (sensitive vs. insensitive). As a single component, PPD-D1 had the most important role (28.0 % of the phenotypic variance), followed by PPD-B1 (12.3 % for PPD-B1_overall, and 15.1 % for PPD-B1_intercopy) and VRN-D1 (2.2 %). Significant gene interactions were identified between the marker alleles within PPD-B1 and between VRN-D1 and the two PPD1 genes. The earliest heading genotypes were those with the photoperiod-insensitive allele in PPD-D1 and PPD-B1, and with the spring allele for VRN-D1 and the winter alleles for VRN-A1 and VRN-B1. This combination could only be detected in genotypes from Southern Europe and Asia. Late-heading genotypes had the sensitivity alleles for both PPD1 genes, regardless of the allelic composition of the VRN1 genes. There was a 10-day difference in heading between the earliest and latest groups under field conditions. The online version of this article (doi:10.1007/s11032-014-0034-2) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
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