Multi-environment genome -wide association mapping of culm morphology traits in barley.
Multi-environment genome -wide association mapping of culm morphology traits in barley.
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DOI：
10.3389/fpls.2022.926277
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
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在节间中空的谷类中，抗倒伏性受节间直径和茎壁厚度等形态特征的影响。尽管它们具有相关性，但在大麦等温带谷类作物中，缺乏对这些性状的遗传控制及其与倒伏关系的了解。为了填补这一空白，我们开发了一种基于图像分析的协议，以准确地对261份大麦材料的茎径和茎壁厚度进行表型分析。对从7个不同环境中的田间试验收集的茎秆性状数据的分析表明，除厚度和硬度外，大多数性状的遗传力值都很高(>；50%)，以及不同环境之间的基因型互作。该种质主要按行型构建，表型相关表明，行型对茎秆性状有混杂效应。在两个行型亚群中，外径和断面模数与倒伏性呈显著负相关(−分别为0.52和0.45)，但与株高不相关，表明有可能独立于株高提高抗倒性。利用50k的iSelect SNP基因分型数据，采用混合模型方法，在整个面板和行型子集上进行了多环境全基因组关联研究：共发现192个数量性状基因座(QTL)，其中包括亚群体特有的QTL和21个影响茎粗和/或断面模数的主效基因座，它们在不影响株高的情况下影响倒伏。这项工作提供了对大麦茎秆形态遗传结构的洞察，以及与激素和细胞壁相关途径相关的候选基因的可能作用，支持了支撑秸秆特征的基因座在不断变化的环境下改善大麦抗倒伏和提高大麦产量稳定性的潜力。
In cereals with hollow internodes, lodging resistance is influenced by morphological characteristics such as internode diameter and culm wall thickness. Despite their relevance, knowledge of the genetic control of these traits and their relationship with lodging is lacking in temperate cereals such as barley. To fill this gap, we developed an image analysis–based protocol to accurately phenotype culm diameters and culm wall thickness across 261 barley accessions. Analysis of culm trait data collected from field trials in seven different environments revealed high heritability values (>50%) for most traits except thickness and stiffness, as well as genotype-by-environment interactions. The collection was structured mainly according to row-type, which had a confounding effect on culm traits as evidenced by phenotypic correlations. Within both row-type subsets, outer diameter and section modulus showed significant negative correlations with lodging (<−0.52 and <−0.45, respectively), but no correlation with plant height, indicating the possibility of improving lodging resistance independent of plant height. Using 50k iSelect SNP genotyping data, we conducted multi-environment genome-wide association studies using mixed model approach across the whole panel and row-type subsets: we identified a total of 192 quantitative trait loci (QTLs) for the studied traits, including subpopulation-specific QTLs and 21 main effect loci for culm diameter and/or section modulus showing effects on lodging without impacting plant height. Providing insights into the genetic architecture of culm morphology in barley and the possible role of candidate genes involved in hormone and cell wall–related pathways, this work supports the potential of loci underpinning culm features to improve lodging resistance and increase barley yield stability under changing environments.
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影响因子：
5.8
作者：
Berry, P. M.;Spink, J.
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