Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering.
Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering.
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DOI：
10.3390/bios11070234
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发表时间：
2021-07-11
期刊：
影响因子：
--
通讯作者：
Zughaier SM
中科院分区：
文献类型：
--
作者：
Wu X;Zhao Y;Zughaier SM
细菌内毒素是细菌在生长和感染过程中不断脱落的脂多糖（lps），是革兰氏阴性细菌外膜小叶的主要成分，也是一种特征明确的TLR4-MD-2配体。我们首次报道了大肠杆菌、鼠伤寒沙门氏菌、明尼苏达沙门氏菌、霍乱弧菌、根瘤菌R. CE3和R. NGR肠道lps的独特表面增强拉曼散射（SERS）光谱，以及脑膜炎奈瑟菌内毒素结构、lps、脂质A和kdo2 -脂质A。SERS光谱的特征峰显示，大部分被测LPS结构来自脂质和糖类，即LPS的主要成分，这些光谱可以成功地用于主成分分析的内毒素区分。此外，本文中所有LPS样品的检测浓度均为10 nmol /mL，这与它们在临床感染中的相关病理生理浓度相对应。本研究表明，lps可以作为生物标志物，用于基于sers的细菌高灵敏度检测。
Bacterial endotoxins, as major components of Gram-negative bacterial outer membrane leaflets and a well-characterized TLR4-MD-2 ligand, are lipopolysaccharides (LPSs) that are constantly shed from bacteria during growth and infection. For the first time, we report that unique surface-enhanced Raman scattering (SERS) spectra of enteric LPSs from E. coli, S. typhimurium, S. minnesota, V. cholerae, Rhizobium species R. CE3, and R. NGR, as well as Neisseria meningitidis endotoxin structures, LPSs, lipid A, and KDO2-lipid A can be obtained. The characteristic peaks of the SERS spectra reveal that most of the tested LPS structures are from lipids and saccharides, i.e., the major components of LPSs, and these spectra can be successfully used to differentiate between endotoxins with principal components analysis. In addition, all the LPS samples here are measured at a concentration of 10 nmole/mL, which corresponds to their relevant pathophysiological concentrations in clinical infections. This study demonstrates that LPSs can be used as biomarkers for the highly sensitive detection of bacteria using SERS-based methods.
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