Blue and red in the protein world: Photoactive yellow protein and phytochromes as revealed by time-resolved crystallography.
Blue and red in the protein world: Photoactive yellow protein and phytochromes as revealed by time-resolved crystallography.
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DOI：
10.1063/4.0000233
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发表时间：
2024-01
影响因子：
2.8
通讯作者：
Stojkovic, Emina A.
中科院分区：
文献类型：
--
作者：
Schmidt, Marius;Stojkovic, Emina A.
时间分辨晶体学（TRX）是一种旨在研究生物大分子在所有时间尺度上的功能运动的方法。 TRX 最初是一种基于同步加速器的方法，后来通过 TR Laue 晶体学 (TRLX) 的发展得以实现。 TR系列晶体学（TR-SX）是TRLX的扩展。随着 TRLX 的基础从 20 世纪 80 年代末到世纪之交不断发展，TR-SX 受到了硬 X 射线自由电子激光器的发展的启发。极强、超短的 X 射线脉冲可以探测微米和纳米晶体，但与此同时，它们会造成辐射损伤，需要更换新晶体。因此，大量的微晶体以连续的方式一一暴露在 X 射线下。通过 TR-SX 方法，可以克服以前方法的最大障碍之一，即与非循环（不可逆）反应研究相关的难以克服的挑战。本文介绍了 Keith Moffat 及其合作者在 TRX 领域取得的成功和变革性贡献，重点介绍了 Moffat 实验室在世纪之交发起的两个关于蛋白质光感受器的重大项目。
Time-resolved crystallography (TRX) is a method designed to investigate functional motions of biological macromolecules on all time scales. Originally a synchrotron-based method, TRX is enabled by the development of TR Laue crystallography (TRLX). TR serial crystallography (TR-SX) is an extension of TRLX. As the foundations of TRLX were evolving from the late 1980s to the turn of the millennium, TR-SX has been inspired by the development of Free Electron Lasers for hard X-rays. Extremely intense, ultrashort x-ray pulses could probe micro and nanocrystals, but at the same time, they inflicted radiation damage that necessitated the replacement by a new crystal. Consequently, a large number of microcrystals are exposed to X-rays one by one in a serial fashion. With TR-SX methods, one of the largest obstacles of previous approaches, namely, the unsurmountable challenges associated with the investigation of non-cyclic (irreversible) reactions, can be overcome. This article describes successes and transformative contributions to the TRX field by Keith Moffat and his collaborators, highlighting two major projects on protein photoreceptors initiated in the Moffat lab at the turn of the millennium.
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影响因子：
48
作者：
Fuller FD;Gul S;Chatterjee R;Burgie ES;Young ID;Lebrette H;Srinivas V;Brewster AS;Michels-Clark T;Clinger JA;Andi B;Ibrahim M;Pastor E;de Lichtenberg C;Hussein R;Pollock CJ;Zhang M;Stan CA;Kroll T;Fransson T;Weninger C;Kubin M;Aller P;Lassalle L;Bräuer P;Miller MD;Amin M;Koroidov S;Roessler CG;Allaire M;Sierra RG;Docker PT;Glownia JM;Nelson S;Koglin JE;Zhu D;Chollet M;Song S;Lemke H;Liang M;Sokaras D;Alonso-Mori R;Zouni A;Messinger J;Bergmann U;Boal AK;Bollinger JM Jr;Krebs C;Högbom M;Phillips GN Jr;Vierstra RD;Sauter NK;Orville AM;Kern J;Yachandra VK;Yano J
通讯作者：
Yano J
影响因子：
4.6
作者：
Edlund P;Takala H;Claesson E;Henry L;Dods R;Lehtivuori H;Panman M;Pande K;White T;Nakane T;Berntsson O;Gustavsson E;Båth P;Modi V;Roy-Chowdhury S;Zook J;Berntsen P;Pandey S;Poudyal I;Tenboer J;Kupitz C;Barty A;Fromme P;Koralek JD;Tanaka T;Spence J;Liang M;Hunter MS;Boutet S;Nango E;Moffat K;Groenhof G;Ihalainen J;Stojković EA;Schmidt M;Westenhoff S
通讯作者：
Westenhoff S
DOI：
10.1126/science.1217737
发表时间：
2012-07-20
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boutet S;Lomb L;Williams GJ;Barends TR;Aquila A;Doak RB;Weierstall U;DePonte DP;Steinbrener J;Shoeman RL;Messerschmidt M;Barty A;White TA;Kassemeyer S;Kirian RA;Seibert MM;Montanez PA;Kenney C;Herbst R;Hart P;Pines J;Haller G;Gruner SM;Philipp HT;Tate MW;Hromalik M;Koerner LJ;van Bakel N;Morse J;Ghonsalves W;Arnlund D;Bogan MJ;Caleman C;Fromme R;Hampton CY;Hunter MS;Johansson LC;Katona G;Kupitz C;Liang M;Martin AV;Nass K;Redecke L;Stellato F;Timneanu N;Wang D;Zatsepin NA;Schafer D;Defever J;Neutze R;Fromme P;Spence JC;Chapman HN;Schlichting I
通讯作者：
Schlichting I
影响因子：
64.8
作者：
Bhoo, SH;Davis, SJ;Vierstra, RD
通讯作者：
Vierstra, RD
影响因子：
56.9
作者：
Genick, UK;Borgstahl, GEO;Getzoff, ED
通讯作者：
Getzoff, ED