Identification of genes and proteins associated with anagen wool growth
Identification of genes and proteins associated with anagen wool growth
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鉴定与毛发生长初期羊毛生长相关的基因和蛋白质
DOI：
10.1111/age.12480
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发表时间：
2017-02
期刊：
影响因子：
2.4
通讯作者：
李和刚
中科院分区：
文献类型：
--
作者：
赵金山;柳楠;刘开东;贺建宁;余娟娟;卜然;程明;德伟;刘积凤;李和刚
确定影响羊毛生长的主要基因，为提高细毛品质和产量提供策略。在这项研究中，我们使用安吉伦绵羊基因表达芯片和蛋白质组学技术，研究了敖汉细毛羊（中国本土品种）毛囊生长阶段体侧皮肤（长毛较多）与腹股沟皮肤（不长毛）的基因表达模式。微阵列研究显示，在体侧皮肤与腹股沟皮肤（S/G）的比较中，4772个探针存在差异表达，包括2071个上调探针和2701个下调探针。通过定量PCR对芯片结果进行验证。共有1099个探针被分配到独特的基因/转录本上。不同基因/转录本数量为926个，其中上调352个，下调574个。在S/G中，13个基因上调10倍以上，60个基因下调10倍以上。进一步分析发现，与羊毛生长有关的基因大部分可归类为细胞分裂调控、中间丝调控、细胞骨架调控和生长因子活性调控。几个潜在的基因家族可能参与头发生长调控，包括成纤维细胞生长因子、转化生长因子-β、WNTs、胰岛素样生长因子、血管内皮生长因子等。蛋白质组学分析还发现196个差异表达蛋白点，其中121个为单蛋白点。
Identifying genes of major effect for wool growth would offer strategies for improving the quality and increasing the yield of fine wool. In this study, we employed the Agilent Sheep Gene Expression Microarray and proteomic technology to investigate the gene expression patterns of body side skin (more wool growing) in Aohan fine wool sheep (a Chinese indigenous breed) in comparison with groin skin (no wool growing) at the anagen stage of the wool follicle. A microarray study revealed that 4772 probes were differentially expressed, including 2071 upregulated and 2701 downregulated probes, in the comparisons of body side skin vs. groin skin (S/G). The microarray results were verified by means of quantitative PCR. A total of 1099 probes were assigned to unique genes/transcripts. The number of distinct genes/transcripts (annotated) was 926, of which 352 were upregulated and 574 were downregulated. In S/G, 13 genes were upregulated by more than 10 fold, whereas 60 genes were downregulated by more than 10 fold. Further analysis revealed that the majority of the genes possibly related to the wool growth could be assigned to categories including regulation of cell division, intermediate filament, cytoskeletal part and growth factor activity. Several potential gene families may participate in hair growth regulation, including fibroblast growth factors, transforming growth factor-β, WNTs, insulin-like growth factor, vascular endothelial growth factors and so on. Proteomic analysis also revealed 196 differentially expressed protein points, of which 121 were identified as single protein points.
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影响因子：
7
作者：
Beyer, A;Hollunder, J;Wilhelm, T
通讯作者：
Wilhelm, T
DOI：
10.1186/1297-9686-37-s1-s97
发表时间：
2005
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Purvis IW;Franklin IR
通讯作者：
Franklin IR
DOI：
10.1016/s0093-3619(08)70911-0
发表时间：
2008
期刊：
Yearbook of Dermatology and Dermatologic Surgery
影响因子：
--
作者：
B. Thiers
通讯作者：
B. Thiers
天真的绵羊的基因表达分析在遗传上具有抗性，并且对胃肠道线虫敏感。
影响因子：
4.4
作者：
Keane, OM;Zadissa, A;Wilson, T;Hyndman, DL;Greer, GJ;Baird, DB;McCulloch, AF;Crawford, AM;McEwan, JC
通讯作者：
McEwan, JC
影响因子：
2.6
作者：
Smith WJ;Li Y;Ingham A;Collis E;McWilliam SM;Dixon TJ;Norris BJ;Mortimer SI;Moore RJ;Reverter A
通讯作者：
Reverter A