A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep.
A genomics-informed, SNP association study reveals FBLN1 and FABP4 as contributing to resistance to fleece rot in Australian Merino sheep.
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DOI：
10.1186/1746-6148-6-27
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发表时间：
2010-05-26
影响因子：
2.6
通讯作者：
Reverter A
中科院分区：
文献类型：
--
作者：
Smith WJ;Li Y;Ingham A;Collis E;McWilliam SM;Dixon TJ;Norris BJ;Mortimer SI;Moore RJ;Reverter A
绵毛蝇Lucilia cuprina对美利奴羊的羊毛腐烂和身体攻击是澳大利亚羊毛行业的主要问题，由于管理成本的增加和羊毛产量和质量的下降，造成了巨大的损失。除对羊毛品质有直接影响外，羊毛腐烂也是飞蝇侵袭绵羊身体的主要易感因素。为了研究羊绒腐病抗性的遗传驱动因素，我们构建了一个由近12,000个探针组成的羊-牛联合基因芯片，其中包括6,125个皮肤表达序列标签和5,760个匿名克隆，这些克隆来自对羊绒腐病抗性和感病动物的皮肤消减文库。利用该微阵列平台对6个抗性和6个敏感动物的皮肤样本在FR诱导前、诱导期间和诱导后的基因表达变化进行了分析。使用混合模型方程对数据进行归一化处理，发现155个差异表达基因(DE)。选择了10个DE基因在独立的皮肤样本上进行实时聚合酶链式反应验证。对另外5个DE基因的基因组区域进行了调查，以确定单核苷酸多态(SNP)，这些SNP在三个群体中进行了基因分型，以确定它们与羊毛腐烂抗性的关联。大多数DE基因来源于羊毛腐烂消减文库，其中具有代表性的基因本体论术语包括对细菌的防御反应和表皮发育，这表明这些过程在调控绵羊对羊毛腐烂的反应中发挥了作用。我们专注于促进皮肤物理屏障功能的基因，包括角蛋白、胶原蛋白、纤维蛋白和脂蛋白，以确定与羊毛腐烂评分相关的SNPs。我们确定FBLN1(纤维蛋白)和FABP4(脂肪酸结合蛋白4)是绵羊对羊毛腐烂抗性的关键因素。这些标记在其他群体中的验证可能导致标记辅助选择的重要测试，最终将提高美利奴绵羊的天然羊毛腐烂抗性。
Fleece rot (FR) and body-strike of Merino sheep by the sheep blowfly Lucilia cuprina are major problems for the Australian wool industry, causing significant losses as a result of increased management costs coupled with reduced wool productivity and quality. In addition to direct effects on fleece quality, fleece rot is a major predisposing factor to blowfly strike on the body of sheep. In order to investigate the genetic drivers of resistance to fleece rot, we constructed a combined ovine-bovine cDNA microarray of almost 12,000 probes including 6,125 skin expressed sequence tags and 5,760 anonymous clones obtained from skin subtracted libraries derived from fleece rot resistant and susceptible animals. This microarray platform was used to profile the gene expression changes between skin samples of six resistant and six susceptible animals taken immediately before, during and after FR induction. Mixed-model equations were employed to normalize the data and 155 genes were found to be differentially expressed (DE). Ten DE genes were selected for validation using real-time PCR on independent skin samples. The genomic regions of a further 5 DE genes were surveyed to identify single nucleotide polymorphisms (SNP) that were genotyped across three populations for their associations with fleece rot resistance. The majority of the DE genes originated from the fleece rot subtracted libraries and over-representing gene ontology terms included defense response to bacterium and epidermis development, indicating a role of these processes in modulating the sheep's response to fleece rot. We focused on genes that contribute to the physical barrier function of skin, including keratins, collagens, fibulin and lipid proteins, to identify SNPs that were associated to fleece rot scores. We identified FBLN1 (fibulin) and FABP4 (fatty acid binding protein 4) as key factors in sheep's resistance to fleece rot. Validation of these markers in other populations could lead to vital tests for marker assisted selection that will ultimately increase the natural fleece rot resistance of Merino sheep.
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影响因子：
4
作者：
Colditz, IG;Eisemann, CH;Husband, AJ
通讯作者：
Husband, AJ
影响因子：
3.3
作者：
Genissel, A;Pastinen, T;Long, AD
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Long, AD
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4.4
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Adelson, DL;Cam, GR;Franklin, IR
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Franklin, IR
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CHIN, JC;WATTS, JE
通讯作者：
WATTS, JE
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MCGUIRK, BJ;ATKINS, KD
通讯作者：
ATKINS, KD