Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital.
Characterization of the genetic environment of the blaKPC-2 gene among Klebsiella pneumoniae isolates from a Chinese Hospital.
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DOI：
10.1016/j.bjid.2016.04.003
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发表时间：
2016-07
影响因子：
3.4
通讯作者：
Jiang, Xiaofei
中科院分区：
文献类型：
--
作者：
Shen, Pinghua;Zhang, Ying;Li, Gang;Jiang, Xiaofei
耐碳青霉烯类肺炎克雷伯菌引起的感染已成为主要的医疗威胁，KPC-2酶是介导克雷伯菌对碳青霉烯类抗生素耐药的主导因子。肺炎。本研究旨在确定blaKPC-2的遗传环境，该基因在临床K.在中国上海华山医院回收的肺炎分离株。本研究通过blaKPC-2筛选纳入42株临床分离株。在多位点序列分型和基于PCR的复制子分型（PBRT）的质粒分析之后，使用连接PCR、作图PCR和交叉PCR测定、引物步移和扩增子测序来分析blaKPC-2基因的遗传环境。ST 423、ST 65、ST 977和ST 11在产KPC-2的K.肺炎。发现了两种携带blaKPC-2的遗传结构：Tn 1721-blaKPC-2-Tn 3和Tn 1721-blaKPC-2-Δ Tn 3-IS 26;并分别携带在IncX和IncFII质粒中。因此，blaKPC-2基因的遗传环境具有多样性，Tn 1721-blaKPC-2-Δ Tn 3-IS 26在临床克雷伯氏菌中占优势。肺炎分离株。这项研究揭示了一些遗传环境，并应促进进一步研究blaKPC-2传播的机制。
Infection caused by carbapenem-resistant Klebsiella pneumoniae has become a major healthcare threat and KPC-2 enzyme is a dominant factor mediating carbapenems resistance in K. pneumoniae. This study was designed to determine the genetic environment of blaKPC-2, which prevailed in clinical K. pneumoniae isolates recovered in Huashan Hospital, Shanghai, China. Forty-two clinical isolates were included in this study by blaKPC-2 screening. After multilocus sequence typing and plasmid analyses of PCR-based replicon typing (PBRT), junction PCR, mapping PCR and crossing PCR assays, primer walking, and amplicon sequencing were used to analyze the genetic environment of the blaKPC-2 gene. ST423, ST65, ST977, and ST11 were all detected in KPC-2-producing K. pneumoniae. Two types of blaKPC-2-bearing genetic structure were found: Tn1721-blaKPC-2-Tn3 and Tn1721-blaKPC-2-ΔTn3-IS26; and were carried in IncX and IncFII plasmids, respectively. In conclusion, the genetic environment of the blaKPC-2 gene was diverse and Tn1721-blaKPC-2-ΔTn3-IS26 was dominant in clinical K. pneumoniae isolates in Huashan Hospital. This study sheds some light on the genetic environment and should foster further studies about the mechanism of the blaKPC-2 dissemination.
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影响因子：
5.2
作者：
Chen, Ying-Tsong;Lin, Jung-Chung;Siu, L. Kristopher
通讯作者：
Siu, L. Kristopher
影响因子：
4.9
作者：
Woodford, N;Tierno, PM;Livermore, DM
通讯作者：
Livermore, DM
影响因子：
5.2
作者：
Shin, Juyoun;Ko, Kwan Soo
通讯作者：
Ko, Kwan Soo
影响因子：
4.9
作者：
Jiang, Yan;Yu, Dongliang;Yu, Yunsong
通讯作者：
Yu, Yunsong
DOI：
10.1016/s1473-3099(13)70190-7
发表时间：
2013-09
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Munoz-Price LS;Poirel L;Bonomo RA;Schwaber MJ;Daikos GL;Cormican M;Cornaglia G;Garau J;Gniadkowski M;Hayden MK;Kumarasamy K;Livermore DM;Maya JJ;Nordmann P;Patel JB;Paterson DL;Pitout J;Villegas MV;Wang H;Woodford N;Quinn JP
通讯作者：
Quinn JP