Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria.
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DOI：
10.1021/acs.chemrev.1c00055
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发表时间：
2021-05-12
期刊：
影响因子：
62.1
通讯作者：
Bavro VN
中科院分区：
文献类型：
--
作者：
Alav I;Kobylka J;Kuth MS;Pos KM;Picard M;Blair JMA;Bavro VN
革兰氏阴性菌中的三方外排泵和相关的1型分泌系统（T1 SS）在功能、结构和组织上是多样的。它们形成跨越内膜和外膜的连续管道，并由三个主要成分组成-通电的内膜转运蛋白（属于ABC，RND和MFS家族），外膜因子通道样蛋白，以及连接两者的周质衔接蛋白（PAP），也称为膜融合蛋白（MFP）。在这篇综述中，我们总结了最新的进展，了解这些系统的结构生物学，功能和调节，突出了以前未描述的作用，PAP在提供一个共同的建筑支架在不同的家庭的转运。尽管是从有限数量的基本结构域构建的，但这些复合体呈现出惊人的各种架构。虽然从RND转运体系统中获得了关键的见解，但对不同三方系统的操作和结构组织的仔细检查揭示了它们之间意想不到的相似性，包括围绕MFS和ATP驱动的转运体形成的相似性，这表明它们围绕基本的共同原则运作。在此基础上，我们提出了一个新的整合模型的PAP介导的通信内的构象循环的三方系统，它可以扩展到其他类型的组件。
Tripartite efflux pumps and the related type 1 secretion systems (T1SSs) in Gram-negative organisms are diverse in function, energization, and structural organization. They form continuous conduits spanning both the inner and the outer membrane and are composed of three principal components—the energized inner membrane transporters (belonging to ABC, RND, and MFS families), the outer membrane factor channel-like proteins, and linking the two, the periplasmic adaptor proteins (PAPs), also known as the membrane fusion proteins (MFPs). In this review we summarize the recent advances in understanding of structural biology, function, and regulation of these systems, highlighting the previously undescribed role of PAPs in providing a common architectural scaffold across diverse families of transporters. Despite being built from a limited number of basic structural domains, these complexes present a staggering variety of architectures. While key insights have been derived from the RND transporter systems, a closer inspection of the operation and structural organization of different tripartite systems reveals unexpected analogies between them, including those formed around MFS- and ATP-driven transporters, suggesting that they operate around basic common principles. Based on that we are proposing a new integrated model of PAP-mediated communication within the conformational cycling of tripartite systems, which could be expanded to other types of assemblies.
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影响因子：
4.2
作者：
Adebusuyi AA;Foght JM
通讯作者：
Foght JM
影响因子：
5.3
作者：
Abdali N;Parks JM;Haynes KM;Chaney JL;Green AT;Wolloscheck D;Walker JK;Rybenkov VV;Baudry J;Smith JC;Zgurskaya HI
通讯作者：
Zgurskaya HI
影响因子：
3.2
作者：
Akatsuka, H;Binet, R;Omori, K
通讯作者：
Omori, K
DOI：
10.1126/science.1168750
发表时间：
2009-03-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Aller SG;Yu J;Ward A;Weng Y;Chittaboina S;Zhuo R;Harrell PM;Trinh YT;Zhang Q;Urbatsch IL;Chang G
通讯作者：
Chang G
影响因子：
3.2
作者：
Akeda, Y;Galán, JE
通讯作者：
Galán, JE