Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay.

Carbon Nanotubes as Fluorescent Labels for Surface Plasmon Resonance-Assisted Fluoroimmunoassay.
复制标题

碳纳米管作为表面等离子体共振辅助荧光免疫测定的荧光标签。

DOI:
10.3390/s17112569
复制
发表时间:
2017-11-07
期刊:
Sensors (Basel, Switzerland)
影响因子:
--
通讯作者:
Fujimaki M
Fujimaki M
中科院分区:
其他
文献类型:
--
作者:
Ashiba H;Iizumi Y;Okazaki T;Wang X;Fujimaki M

文献摘要

参考文献

被引文献

相似文献

碳纳米管的光致发光特性,包括大的斯托克斯位移和没有荧光光漂白,可以用作生物测量中的荧光标记。在这项研究中,碳纳米管作为荧光标记的表面等离子体共振(SPR)辅助荧光免疫分析的性能进行了评估。使用配备有棱镜集成微流体通道的传感器芯片来激发SPR,观察具有670 nm的激发波长和970 nm的发射波长的(8,3)CNT的荧光。使用可见光照相机分散在水中的CNT的最小可检测浓度为0.25 μg/mL,相当于2 × 1010管/mL。通过评估检测体积中CNT的可检测数量,从理论上研究了使用CNT荧光标记的目标分析物检测。假设检测到与100个CNT标记物结合的病毒颗粒,则可检测病毒颗粒的最小数量计算为900。结果表明,碳纳米管是一种有效的SPR辅助免疫荧光标记物。
The photoluminescence properties of carbon nanotubes (CNTs), including the large Stokes shift and the absence of fluorescent photobleaching, can be used as a fluorescent label in biological measurements. In this study, the performance of CNTs as a fluorescent label for surface plasmon resonance (SPR)-assisted fluoroimmunoassay is evaluated. The fluorescence of (8, 3) CNTs with an excitation wavelength of 670 nm and an emission wavelength of 970 nm is observed using a sensor chip equipped with a prism-integrated microfluidic channel to excite the SPR. The minimum detectable concentration of a CNT dispersed in water using a visible camera is 0.25 μg/mL, which is equivalent to 2 × 1010 tubes/mL. The target analyte detection using the CNT fluorescent labels is theoretically investigated by evaluating the detectable number of CNTs in a detection volume. Assuming detection of virus particles which are bound with 100 CNT labels, the minimum number of detectable virus particles is calculated to be 900. The result indicates that CNTs are effective fluorescent labels for SPR-assisted fluoroimmunoassay.
DOI: 10.1126/science.1072631
发表时间: 2002-07-26
期刊: SCIENCE
影响因子: 56.9
作者:
O'Connell, MJ;Bachilo, SM;Smalley, RE
通讯作者: Smalley, RE
DOI: 10.1016/s0927-7757(99)00550-6
发表时间: 2000-10-10
影响因子: 5.2
作者:
Liebermann, T;Knoll, W
通讯作者: Knoll, W
DOI: 10.1021/ja071553d
发表时间: 2007-07-04
影响因子: 15
作者:
Crochet, Jared;Clemens, Michael;Hertel, Tobias
通讯作者: Hertel, Tobias
DOI: 10.1021/la025578w
发表时间: 2002-08-06
期刊: LANGMUIR
影响因子: 3.9
作者:
Roy, S;Kim, JH;Gast, AP
通讯作者: Gast, AP
DOI: 10.1007/s12274-009-9009-8
发表时间: 2009-02-01
期刊: NANO RESEARCH
影响因子: 9.9
作者:
Liu, Zhuang;Tabakman, Scott;Welsher, Kevin;Dai, Hongjie
通讯作者: Dai, Hongjie