Phthalocyanine dimerization-based molecular beacons using near-IR fluorescence.
Phthalocyanine dimerization-based molecular beacons using near-IR fluorescence.
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DOI：
10.1021/ja8088247
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发表时间：
2009-02-25
影响因子：
15
通讯作者：
Soper SA
中科院分区：
文献类型：
--
作者：
Nesterova IV;Erdem SS;Pakhomov S;Hammer RP;Soper SA
Herein we demonstrate the use of a novel dimerization-based molecular beacon (MB) probe consisting of two metallo-phthalocyanine (Pc) fluorophores that use near-IR fluorescence, appropriate for highly specific and sensitive in-vivo and/or in-vitro DNA/RNA detection. PC 具有形成非荧光 H-二聚体的倾向，该二聚体被用作闭合 MB 构象中的分子“关闭”开关。 The “on” switch, which is generated when the solution target binds to the loop of the MB forming the open form, also provides two fluorophores for transduction resulting in a doubling of the extinction coefficient and improving the resulting fluorescence yield compared to a classical single-fluorophore/quencher MB system. In addition, the Pc-based MBs possess high thermal, photo and chemical stabilities that are essential for many highly sensitive applications, such as molecular imaging. The dimer-based MBs were obtained using a simple single-step synthesis procedure and demonstrated excellent quenching efficiencies (98%) as well as a high signal-to-background ratio (~60) exceeding the performance characteristics of many conventionally-available MB probes.
Herein we demonstrate the use of a novel dimerization-based molecular beacon (MB) probe consisting of two metallo-phthalocyanine (Pc) fluorophores that use near-IR fluorescence, appropriate for highly specific and sensitive in-vivo and/or in-vitro DNA/RNA detection. Pc’s possess a propensity to form non-fluorescent H-dimers that is utilized as the molecular “off” switch in the closed MB conformation. The “on” switch, which is generated when the solution target binds to the loop of the MB forming the open form, also provides two fluorophores for transduction resulting in a doubling of the extinction coefficient and improving the resulting fluorescence yield compared to a classical single-fluorophore/quencher MB system. In addition, the Pc-based MBs possess high thermal, photo and chemical stabilities that are essential for many highly sensitive applications, such as molecular imaging. The dimer-based MBs were obtained using a simple single-step synthesis procedure and demonstrated excellent quenching efficiencies (98%) as well as a high signal-to-background ratio (~60) exceeding the performance characteristics of many conventionally-available MB probes.
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影响因子：
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作者：
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通讯作者：
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影响因子：
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