AtROP1 negatively regulates potato resistance to Phytophthora infestans via NADPH oxidase-mediated accumulation of H2O2.
AtROP1 negatively regulates potato resistance to Phytophthora infestans via NADPH oxidase-mediated accumulation of H2O2.
复制标题
AtROP1 通过 NADPH 氧化酶介导的 H2O2 积累负调节马铃薯对致病疫霉的抗性
DOI：
10.1186/s12870-014-0392-2
复制
发表时间：
2014-12-30
影响因子：
5.3
通讯作者：
Zhao J
中科院分区：
文献类型：
--
作者：
Zhang Z;Yang F;Na R;Zhang X;Yang S;Gao J;Fan M;Zhao Y;Zhao J
背景：小gtpase是单体鸟嘌呤核苷酸结合蛋白。在植物中，ROPs调节植物细胞极性、植物细胞分化和发育以及生物和非生物胁迫信号通路。结果：我们报道了利用GFP融合在烟草表皮细胞的质膜上定位AtRop1蛋白的亚细胞定位。此外，马铃薯中拟南芥AtRop1的显性阴性形式（DN）的短暂和稳定表达导致H2O2积累，与Bary山疫霉菌的发育减少和受感染马铃薯叶片上的较小病变有关。采用RT-PCR方法对马铃薯NADPH氧化酶同源基因Strboh-D的表达进行了分析。该基因在转DN-AtRop1基因的植物中被感染后仍能保持表达。分析了水杨酸（SA）和茉莉酸（JA）标记基因Npr1和Lox的转录水平。在DN-AtRop1转基因植株中，Lox基因被显著诱导，而SA上调的Npr1基因的表达在感染后略有下降。结论：本研究结果表明，DN-AtROP1影响马铃薯对病原菌的抗性。这与NADPH氧化酶介导的H2O2产生和JA信号的增加有关。
Background:Small GTPases are monomeric guanine nucleotide-binding proteins. In plants, ROPs regulate plant cell polarity, plant cell differentiation and development as well as biotic and abiotic stress signaling pathways.Results:We report the subcellular localization of the AtRop1 protein at the plasma membrane in tobacco epidermal cells using GFP fusions. Additionally, transient and stable expression of a dominant negative form (DN) of the Arabidopsis AtRop1 in potato led to H2O2 accumulation associated with the reduced development of Phytophthora infestans Montagne de Bary and smaller lesions on infected potato leaves. The expression of the Strboh-D gene, a NADPH oxidase homologue in potato, was analyzed by RT-PCR. Expression of this gene was maintained in DN-AtRop1 transgenic plants after infection with P. infestans. In transgenic potato lines, the transcript levels of salicylic acid (SA) and jasmonic acid (JA) marker genes (Npr1 and Lox, respectively) were analyzed. The Lox gene was induced dramatically whereas expression of Npr1, a gene up-regulated by SA, decreased slightly in DN-AtRop1 transgenic plants after infection with P. infestans.Conclusions:In conclusion, our results indicate that DN-AtROP1 affects potato resistance to P. infestans. This is associated with increased NADPH oxidase-mediated H2O2 production and JA signaling.
登录
查看更多内容
影响因子：
11.8
作者：
Choi, Jaemyung;Huh, Sung Un;Hwang, Ildoo
通讯作者：
Hwang, Ildoo
影响因子：
5.3
作者：
Divi UK;Rahman T;Krishna P
通讯作者：
Krishna P
影响因子：
3.9
作者：
Chen, Xu;Friml, Juri
通讯作者：
Friml, Juri
DOI：
10.1016/0048-4059(83)90019-x
发表时间：
1983-01-01
期刊：
PHYSIOLOGICAL PLANT PATHOLOGY
影响因子：
--
作者：
DOKE, N
通讯作者：
DOKE, N
影响因子：
11.6
作者：
Hoefle, Caroline;Huesmann, Christina;Hueckelhoven, Ralph
通讯作者：
Hueckelhoven, Ralph