The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis.
The FtsH-Inactive Protein FtsHi5 Is Required for Chloroplast Development and Protein Accumulation in Chloroplasts at Low Ambient Temperature in Arabidopsis.
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FtsH失活蛋白FtsHi5是拟南芥叶绿体发育和低环境温度下叶绿体蛋白积累所必需的
DOI：
10.3389/fpls.2021.830390
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发表时间：
2021
影响因子：
5.6
通讯作者：
Liu JX
中科院分区：
文献类型：
--
作者：
Li JY;Sun JL;Tian YY;Liu JX
叶绿体是高等植物不可缺少的。植物的生长发育对环境温度的变化非常敏感，叶绿体的发育也受到不利的环境温度的调节。然而，植物如何协调叶绿体发育和环境温度变化的分子机制仍然很大程度上未知。本研究通过正向遗传筛选获得了拟南芥叶色2号温度调节叶绿体发育缺陷突变体热敏突变体(tsl2)。 tsl2突变体在正常生长温度（22℃）下表现出弱黄色表型，在低生长温度（16℃）下该表型更加明显，在高生长温度（29℃）下大部分得到挽救。批量分离分析 (BSA) 显示 TSL2 编码 FtsH-失活蛋白 5 (FtsHi5)。遗传互补分析证实，FtsHi5 的互补表达可以挽救 16°C 下 tsl2 突变体中观察到的叶绿素含量和类囊体发育缺陷。采用串联质量标签（TMT）同量异位标记的定量质谱分析揭示了低温下 tsl2 突变植物叶绿体蛋白质组的广泛变化，这与 tsl2 植物中叶绿体生物发生和功能受损相一致。总之，我们的数据表明 FtsHi5/TSL2 在叶绿体发育和叶绿体蛋白质积累中发挥重要作用，特别是在拟南芥的低环境温度下。
Chloroplasts are indispensable for higher plants. The growth and development of plants are very sensitive to environmental temperature changes, and chloroplast development is also regulated by adverse environmental temperatures. However, the molecular mechanism of how plants coordinate chloroplast development and environmental temperature changes remains largely unknown. Here, a temperature-conditioned chloroplast development defective mutant thermo-sensitive mutant in leaf color 2 (tsl2) of Arabidopsis was obtained through a forward genetic screening. The tsl2 mutant showed a weak yellowish phenotype at normal growth temperature (22°C), and the phenotype was more pronounced at low growth temperature (16°C) and largely rescued at high growth temperature (29°C). Bulk Segregant Analysis (BSA) revealed that TSL2 encodes FtsH-Inactive Protein 5 (FtsHi5). Genetic complementation analysis confirmed that complemented expression of FtsHi5 rescued the chlorophyll content and thylakoid development defects observed in tsl2 mutants at 16°C. Quantitative mass spectrometry analysis with Tandem Mass Tag (TMT) isobaric labeling revealed broad changes in the chloroplast proteome of tsl2 mutant plants at low temperature, which is agreed with the impaired chloroplast biogenesis and function in tsl2 plants. Together, our data demonstrates that FtsHi5/TSL2 plays an important role in chloroplast development and protein accumulation in chloroplasts, especially at low environmental temperatures in Arabidopsis.
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影响因子：
3.7
作者：
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