Diversity in Antagonistic Interactions between Commensal Oral Streptococci and Streptococcus mutans.
Diversity in Antagonistic Interactions between Commensal Oral Streptococci and Streptococcus mutans.
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DOI：
10.1159/000479091
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发表时间：
2018
期刊：
影响因子：
4.2
通讯作者：
Nascimento MM
中科院分区：
文献类型：
--
作者：
Huang X;Browngardt CM;Jiang M;Ahn SJ;Burne RA;Nascimento MM
精氨酸通过口腔细菌的精氨酸脱亚胺酶系统（ADS）代谢产生氨，这可以增加口腔生物膜的pH值，降低龋齿的风险。口腔生物膜中ADS阳性菌和致龋菌之间的拮抗作用可能是龋病的重要生态决定因素。本研究探讨了临床分离的溶血性链球菌对变形链球菌UA159（一种特征良好的致龋人类分离株）的拮抗潜力和机制。戈登链球菌、血链球菌、副血链球菌、南方链球菌和冠状链球菌的低代分离株对S.变形链球菌在先接种或与变形链球菌同时接种的情况下，变种人研究了溶藻菌株对S.在不太丰富的培养基中生长期间或当半乳糖取代葡萄糖作为主要碳水化合物来源时，这些菌株的变形和H2O2的产生得到增强。丙酮酸氧化酶是产H_2O_2的主要途径，但在部分菌株中也检测到乳酸氧化酶活性。gordonii和S.鸡冠。UA159抑制所有测试的溶藻菌株的生长时，首先接种，特别是在有氧条件下。但S.变形链球菌的某些菌株。gordonii和S.在精氨酸存在下的厌氧生长期间未观察到australis。因此，溶龋链球菌可能通过调节生物膜pH值同时拮抗龋齿病原体的生长和毒力而对口腔生物膜的生态学产生协同的积极影响。
Arginine metabolism via the arginine deiminase system (ADS) of oral bacteria generates ammonia, which can increase the pH of oral biofilms and decrease the risk for dental caries. Antagonistic interactions between ADS-positive and cariogenic bacteria in oral biofilms may be an important ecological determinant of caries. This study investigated the antagonistic potential and mechanisms of clinical isolates of arginolytic streptococci on and by Streptococcus mutans UA159, a well-characterized cariogenic human isolate. Low-passage isolates of Streptococcus gordonii, Streptococcus sanguinis, Streptococcus parasanguinis, Streptococcus australis and Streptococcus cristatus inhibited the growth of S. mutans to various degrees when they were inoculated on growth media first or simultaneously with S. mutans. The antagonistic effects of arginolytic strains against S. mutans and the production of H2O2 by these strains were enhanced during growth in a less-rich medium or when galactose was substituted for glucose as the primary carbohydrate source. Pyruvate oxidase was the dominant pathway for H2O2 production by arginolytic strains, but lactate oxidase activity was also detected in some strains of S. gordonii and S. cristatus. UA159 inhibited the growth of all tested arginolytic strains when inoculated first, especially in aerobic conditions. However, the antagonistic effects of S. mutans on certain strains of S. gordonii and S. australis were not observed during anaerobic growth in the presence of arginine. Thus, arginolytic commensal streptococci may have a synergistically positive impact on the ecology of oral biofilms by moderating biofilm pH while antagonizing the growth and virulence of caries pathogens.
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影响因子：
4.2
作者：
Huang X;Schulte RM;Burne RA;Nascimento MM
通讯作者：
Nascimento MM
影响因子：
3.2
作者：
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4.2
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通讯作者：
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3.2
作者：
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通讯作者：
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