Various spatiotemporal expression profiles of anther-expressed genes in rice.
Various spatiotemporal expression profiles of anther-expressed genes in rice.
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DOI：
10.1093/pcp/pcn128
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发表时间：
2008-10
影响因子：
4.9
通讯作者：
Matsuoka, Makoto
中科院分区：
文献类型：
--
作者：
Hobo, Tokunori;Suwabe, Keita;Aya, Koichiro;Suzuki, Go;Yano, Kentaro;Ishimizu, Takeshi;Fujita, Masahiro;Kikuchi, Shunsuke;Hamada, Kazuki;Miyano, Masumi;Fujioka, Tomoaki;Kaneko, Fumi;Kazama, Tomohiko;Mizuta, Yoko;Takahashi, Hirokazu;Shiono, Katsuhiro;Nakazono, Mikio;Tsutsumi, Nobuhiro;Nagamura, Yoshiaki;Kurata, Nori;Watanabe, Masao;Matsuoka, Makoto
雄配子体和绒毡层在花药发育过程中发挥不同的作用，尽管它们来自相同的细胞谱系，即L2层。到目前为止，由于分离这两种细胞类型的技术困难，还不可能描述它们的转录组。在本研究中，我们利用激光显微切割（LM）介导的微阵列对水稻小孢子/花粉和绒毡层的分离转录组进行了表征。 28,141个花药表达基因的时空表达模式被分为20个簇，其中包含3,468个（12.3%）花药富集基因。在一些簇中，观察到同一发育阶段的小孢子和绒毡层中的同步基因表达是花药转录组的新特征。值得注意的表达模式与基因本体（GO）类别和基因注释相关，这些模式与花药发育中的重要生物事件相关，例如花粉成熟、花粉萌发、花粉管伸长和花粉壁形成。
The male gametophyte and tapetum play different roles during anther development although they are differentiated from the same cell lineage, the L2 layer. Until now, it has not been possible to delineate their transcriptomes due to technical difficulties in separating the two cell types. In the present study, we characterized the separated transcriptomes of the rice microspore/pollen and tapetum using laser microdissection (LM)-mediated microarray. Spatiotemporal expression patterns of 28,141 anther-expressed genes were classified into 20 clusters, which contained 3,468 (12.3%) anther-enriched genes. In some clusters, synchronous gene expression in the microspore and tapetum at the same developmental stage was observed as a novel characteristic of the anther transcriptome. Noteworthy expression patterns are discussed in connection with gene ontology (GO) categories and gene annotations, which are related to important biological events in anther development, such as pollen maturation, pollen germination, pollen tube elongation and pollen wall formation.
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影响因子：
11.6
作者：
Fiebig, A;Mayfield, JA;Preuss, D
通讯作者：
Preuss, D
影响因子：
5.1
作者：
Jiang, S;Cai, M;Ramachandran, S
通讯作者：
Ramachandran, S
影响因子：
11.6
作者：
Jung, Ki-Hong;Han, Min-Jung;An, Gynheung
通讯作者：
An, Gynheung
影响因子：
11.6
作者：
Nakazono, M;Qiu, F;Schnable, PS
通讯作者：
Schnable, PS
影响因子：
7.4
作者：
Honys, D;Twell, D
通讯作者：
Twell, D