Expansion microscopy: enabling single cell analysis in intact biological systems.
Expansion microscopy: enabling single cell analysis in intact biological systems.
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DOI：
10.1111/febs.14597
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Boyden ES
中科院分区：
文献类型：
--
作者：
Alon S;Huynh GH;Boyden ES
There is a need for single cell analysis methods that enable the identification and localization of different kinds of biomolecule throughout cells and throughout intact tissues, thereby allowing characterization and classification of individual cells and their relationships to each other within intact systems. Expansion microscopy (ExM) is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution microscopy on diffraction-limited microscopes, enabling rapid image acquisition and large field of view. As a result, ExM is well-positioned to integrate molecular content and cellular morphology, with the spatial precision sufficient to resolve individual biological building blocks, with the scale and accessibility required to deploy over extended 3-D objects like tissues and organs. Expansion microscopy is a technology that physically magnifies tissues in an isotropic way, thereby achieving super-resolution with diffraction-limited microscopes. As a result, expansion microscopy is well-positioned to integrate molecular content and cellular morphology, and can be deployed over extended 3-D objects like tissues and organs. Figure adapted from Tillberg et al., Nat Biotechnol., 2016.
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影响因子：
64.8
作者：
Halpern KB;Shenhav R;Matcovitch-Natan O;Toth B;Lemze D;Golan M;Massasa EE;Baydatch S;Landen S;Moor AE;Brandis A;Giladi A;Avihail AS;David E;Amit I;Itzkovitz S
通讯作者：
Itzkovitz S
影响因子：
19
作者：
Buxbaum AR;Yoon YJ;Singer RH;Park HY
通讯作者：
Park HY
光学成像。膨胀显微镜。
DOI：
10.1126/science.1260088
发表时间：
2015-01-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen F;Tillberg PW;Boyden ES
通讯作者：
Boyden ES
DOI：
10.1073/pnas.1613337113
发表时间：
2016-10-04
影响因子：
11.1
作者：
Crittenden, Jill R.;Tillberg, Paul W.;Graybiel, Ann M.
通讯作者：
Graybiel, Ann M.
影响因子：
48
作者：
Chen F;Wassie AT;Cote AJ;Sinha A;Alon S;Asano S;Daugharthy ER;Chang JB;Marblestone A;Church GM;Raj A;Boyden ES
通讯作者：
Boyden ES