Injectable shear-thinning hydrogels for delivering osteogenic and angiogenic cells and growth factors.
Injectable shear-thinning hydrogels for delivering osteogenic and angiogenic cells and growth factors.
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可注射剪切的水凝胶用于输送成骨和血管生成细胞和生长因子。
DOI：
10.1039/c8bm00293b
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发表时间：
2018-05-29
影响因子：
6.6
通讯作者：
Jang HL
中科院分区：
文献类型：
--
作者：
Alarçin E ;Lee TY ;Karuthedom S ;Mohammadi M ;Brennan MA ;Lee DH ;Marrella A ;Zhang J ;Syla D ;Zhang YS ;Khademhosseini A ;Jang HL
当骨折不稳定或血供受阻时，可能发生骨不连。为了提供一个有效的治疗愈合骨不连缺陷，我们介绍了一种可注射的成骨水凝胶，可以提供细胞和血管生成生长因子。我们使用基于硅酸盐的剪切稀化水凝胶（STH）来设计可注射支架，并掺入聚己内酯（PCL）纳米颗粒，其以受控的方式捕获和释放血管生成生长因子。通过调整STH中明胶和硅酸盐纳米片的固体组成，我们定义了能够注射STH的最佳条件，STH可以递送细胞和生长因子。不同类型的STH可以通过由多个注射器和针头组成的单个头部同时注射到3D结构中，同时以连续的方式保持其工程结构。注射的STH也能够填充骨中任何不规则形状的缺损。将成骨细胞和内皮细胞分别包封在含有和不含血管生成生长因子的STH中，当共培养时，与在单一培养中生长的细胞相比，它们的生长和分化显著增强。本研究介绍了一个新的平台，形状可调的材料与控制释放的血管生成生长因子，利用PCL纳米粒子开发的第一步。
Bone nonunion may occur when the fracture is unstable, or blood supply is impeded. To provide an effective treatment for healing of nonunion defects, we introduce an injectable osteogenic hydrogel that can deliver cells and vasculogenic growth factors. We used a silicate-based shear-thinning hydrogel (STH) to engineer an injectable scaffold and incorporated polycaprolactone (PCL) nanoparticles that entrap and release vasculogenic growth factors in a controlled manner. By adjusting the solid composition of gelatin and silicate nanoplatelets in the STH, we defined optimal conditions that enable injection of STHs, which can deliver cells and growth factors. Different types of STHs could be simultaneously injected into 3D constructs through a single head composed of multiple syringes and needles, while maintaining its engineered structure in a continuous manner. Injected STHs were also capable of filling any irregularly shaped defects in bone. Osteogenic cells and endothelial cells were encapsulated in STHs with and without vasculogenic growth factors respectively, and when co-cultured, their growth and differentiation were significantly enhanced compared to cells grown in monoculture. This study introduces an initial step of developing a new platform of shape tunable material with controlled release of angiogenic growth factors by utilizing PCL nanoparticles.
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影响因子：
2.3
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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