DNA barcoding and species boundary delimitation of selected species of Chinese Acridoidea (Orthoptera: Caelifera).
DNA barcoding and species boundary delimitation of selected species of Chinese Acridoidea (Orthoptera: Caelifera).
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中国蝗总科（直翅目：Caelifera）选定物种的DNA条形码和物种界线界定
DOI：
10.1371/journal.pone.0082400
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Huang J;Zhang A;Mao S;Huang Y
我们测试了DNA条形码在蝗总科的性能，并试图解决物种边界划定问题，在选定的群体使用COI条形码。应用三种分析方法重建了系统发育过程。K2P距离被用来评估种内变异和种间分歧之间的重叠范围。利用“最佳匹配（BM）"、“最佳近似匹配（BLS）"、“所有物种条形码（ASB）”和“反向传播神经网络（BP方法）”测试物种识别的成功率。应用系统发生种概念和网络分析方法对8个种组进行了种界划分。结果表明，COI条形码区域在属和种水平上的系统发育重建效果优于在更高水平上的系统发育重建效果，但在排除第三碱基数据或同时排除第一和第三碱基数据的情况下，COI条形码区域在更高水平上的系统发育重建效果有所改善。大多数重叠和错误鉴定可能是由于分类不完善造成的，这表明分类修订在DNA条形码研究中的关键作用。物种边界划定证实存在过度分裂在6个物种组，并建议每个组应被视为一个单一的物种。
We tested the performance of DNA barcoding in Acridoidea and attempted to solve species boundary delimitation problems in selected groups using COI barcodes. Three analysis methods were applied to reconstruct the phylogeny. K2P distances were used to assess the overlap range between intraspecific variation and interspecific divergence. “Best match (BM)”, “best close match (BCM)”, “all species barcodes (ASB)” and “back-propagation neural networks (BP-based method)” were utilized to test the success rate of species identification. Phylogenetic species concept and network analysis were employed to delimitate the species boundary in eight selected species groups. The results demonstrated that the COI barcode region performed better in phylogenetic reconstruction at genus and species levels than at higher-levels, but showed a little improvement in resolving the higher-level relationships when the third base data or both first and third base data were excluded. Most overlaps and incorrect identifications may be due to imperfect taxonomy, indicating the critical role of taxonomic revision in DNA barcoding study. Species boundary delimitation confirmed the presence of oversplitting in six species groups and suggested that each group should be treated as a single species.
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