Fourier transform infrared imaging microspectroscopy and tissue-level mechanical testing reveal intraspecies variation in mouse bone mineral and matrix composition.
Fourier transform infrared imaging microspectroscopy and tissue-level mechanical testing reveal intraspecies variation in mouse bone mineral and matrix composition.
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DOI：
10.1007/s00223-008-9176-8
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发表时间：
2008-11
影响因子：
4.2
通讯作者：
Jepsen, Karl J.
中科院分区：
文献类型：
--
作者：
Courtland, Hayden-William;Nasser, Philip;Goldstone, Andrew B.;Spevak, Lyudmila;Boskey, Adele L.;Jepsen, Karl J.
骨折的易感性是遗传的，并取决于骨的形态和质量。然而，骨质量的研究通常被强调骨几何形状和骨矿物质密度所掩盖。鉴于矿物和基质成分的差异存在于各种物种中，我们假设骨质量和组织水平机械性能的遗传变异也存在于物种中。采用傅里叶变换红外成像和组织水平力学测试分析了16周龄雌性A/J、C57 BL/6 J（B6）和C3 H/HeJ（C3 H）近交系小鼠股骨的矿物成分、矿物成熟度、胶原交联率和组织水平力学性能的变化。与B6相比，A/J股骨的矿物质-基质比增加。C3 H矿物与基质的比例介于A/J和B6之间。与A/J和B6相比，C3 H股骨的酸性磷酸盐和碳酸盐水平降低，胶原交联率增加。模量值等于矿物-基质值，其中A/J股骨最坚硬，B6最不坚硬，C3 H具有中等硬度。此外，不同菌株的失效工作量不同，高矿化和脆性A/J股骨的失效工作量最少。因此，近交系小鼠能够差异调节其骨矿物质的组成和其骨基质的成熟度以及组织水平的机械性能。这些结果表明，骨质量和形态特征的特定组合是遗传调节的，因此可以以不同的方式构建具有机械功能的骨。
Fracture susceptibility is heritable and dependent upon bone morphology and quality. However, studies of bone quality are typically overshadowed by emphasis on bone geometry and bone mineral density. Given that differences in mineral and matrix composition exist in a variety of species, we hypothesized that genetic variation in bone quality and tissue-level mechanical properties would also exist within species. Sixteen-week-old female A/J, C57BL/6J (B6), and C3H/HeJ (C3H) inbred mouse femora were analyzed using Fourier transform infrared imaging and tissue-level mechanical testing for variation in mineral composition, mineral maturity, collagen cross-link ratio, and tissue-level mechanical properties. A/J femora had an increased mineral-to-matrix ratio compared to B6. The C3H mineral-to-matrix ratio was intermediate of A/J and B6. C3H femora had reduced acid phosphate and carbonate levels and an increased collagen cross-link ratio compared to A/J and B6. Modulus values paralleled mineral-to-matrix values, with A/J femora being the most stiff, B6 being the least stiff, and C3H having intermediate stiffness. In addition, work-to-failure varied among the strains, with the highly mineralized and brittle A/J femora performing the least amount of work-to-failure. Inbred mice are therefore able to differentially modulate the composition of their bone mineral and the maturity of their bone matrix in conjunction with tissue-level mechanical properties. These results suggest that specific combinations of bone quality and morphological traits are genetically regulated such that mechanically functional bones can be constructed in different ways.
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影响因子：
2.9
作者：
Blank, RD;Baldini, TH;Paschalis, EP
通讯作者：
Paschalis, EP
影响因子：
4.5
作者：
BONFIELD, W;CLARK, EA
通讯作者：
CLARK, EA
影响因子：
4.2
作者：
BOSKEY, AL;MARKS, SC
通讯作者：
MARKS, SC
影响因子：
2.4
作者：
Harvey, KB;Donahue, SW
通讯作者：
Donahue, SW
影响因子：
4.1
作者：
Beamer, WG;Donahue, LR;Baylink, DJ
通讯作者：
Baylink, DJ