Combination of genetic analysis and ancient literature survey reveals the divergence of traditional Brassica rapa varieties from Kyoto, Japan.
Combination of genetic analysis and ancient literature survey reveals the divergence of traditional Brassica rapa varieties from Kyoto, Japan.
复制标题
DOI：
10.1038/s41438-021-00569-0
复制
发表时间：
2021-06-01
影响因子：
8.7
通讯作者：
Kimura S
中科院分区：
文献类型：
--
作者：
Kawakatsu Y;Sakamoto T;Nakayama H;Kaminoyama K;Igarashi K;Yasugi M;Kudoh H;Nagano AJ;Yano K;Kubo N;Notaguchi M;Kimura S
自古以来，人类就培育了多种我们今天赖以生存的植物。然而，人们对大多数这些植物的分化知之甚少。在本研究中，我们通过结合遗传分析和古代文献调查，调查了日本京都的传统叶菜水菜（Brassica rapa L. subsp. nipposinica L. H. Bailey）的分歧。 Mibuna 被认为是 200 年前从京都另一种传统叶菜水菜 (Mizuna) 培育出来的。 Mibuna 有简单的匙形叶子，而 Mizuna 有特有的锯齿状叶子。数量性状位点（QTL）和基因表达分析表明，BrTCP15表达下调导致叶形从锯齿状变为简单匙形。有趣的是，SNP 分析表明包含 BrTCP15 基因座的基因组区域通过基因渗入转移到 Mibuna。此外，我们还对古代文献进行了调查，以揭示水菜的分歧，发现水菜和单叶萝卜之间可能在过去发生过杂交。事实上，对多个萝卜品种的基因组分析表明，其中一个品种 Murasakihime 的 BrTCP15 区域与 Mibuna 具有几乎相同的序列。这些结果表明水菜和芜菁之间的杂交导致了水菜的建立。
Since ancient times, humans have bred several plants that we rely on today. However, little is known about the divergence of most of these plants. In the present study, we investigated the divergence of Mibuna (Brassica rapa L. subsp. nipposinica L. H. Bailey), a traditional leafy vegetable in Kyoto (Japan), by combining genetic analysis and a survey of ancient literature. Mibuna is considered to have been bred 200 years ago from Mizuna, another traditional leafy vegetable in Kyoto. Mibuna has simple spatulate leaves, whereas Mizuna has characteristic serrated leaves. The quantitative trait loci (QTL) and gene expression analyses suggested that the downregulation of BrTCP15 expression contributed to the change in the leaf shape from serrated to simple spatulate. Interestingly, the SNP analysis indicated that the genomic region containing the BrTCP15 locus was transferred to Mibuna by introgression. Furthermore, we conducted a survey of ancient literature to reveal the divergence of Mibuna and found that hybridization between Mizuna and a simple-leaved turnip might have occurred in the past. Indeed, the genomic analysis of multiple turnip cultivars showed that one of the cultivars, Murasakihime, has almost the same sequence in the BrTCP15 region as Mibuna. These results suggest that the hybridization between Mizuna and turnip has resulted in the establishment of Mibuna.
登录
查看更多内容
影响因子：
4.6
作者：
Hay, Angela;Barkoulas, Michalis;Tsiantis, Miltos
通讯作者：
Tsiantis, Miltos
DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.7
作者：
Baird NA;Etter PD;Atwood TS;Currey MC;Shiver AL;Lewis ZA;Selker EU;Cresko WA;Johnson EA
通讯作者：
Johnson EA
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro
影响因子：
30.8
作者：
Ori, Naomi;Cohen, Aya Refael;Eshed, Yuval
通讯作者：
Eshed, Yuval