CHESS: a new human gene catalog curated from thousands of large-scale RNA sequencing experiments reveals extensive transcriptional noise.
CHESS: a new human gene catalog curated from thousands of large-scale RNA sequencing experiments reveals extensive transcriptional noise.
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DOI：
10.1186/s13059-018-1590-2
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发表时间：
2018-11-28
期刊：
影响因子：
12.3
通讯作者：
Salzberg SL
中科院分区：
文献类型：
--
作者：
Pertea M;Shumate A;Pertea G;Varabyou A;Breitwieser FP;Chang YC;Madugundu AK;Pandey A;Salzberg SL
我们通过基因型组织表达（GTEx）项目从深度RNA测序实验中组装了序列，以创建一个新的人类基因和转录本目录，称为CHESS。新数据库包含42，611个基因，其中20，352个可能是蛋白质编码基因，22，259个是非编码基因，总共有323，258个转录本。其中包括224个新的蛋白质编码基因和116，156个新的转录本。我们在超过65万个基因组位点检测到超过3000万个额外的转录物，几乎所有这些转录物都可能是无功能的，揭示了人类细胞中迄今为止未被重视的转录噪音量。CHESS数据库可在http://ccb.jhu.edu/chess上获得。本文的在线版本（10.1186/s13059-018-1590-2）包含补充材料，可供授权用户使用。
We assembled the sequences from deep RNA sequencing experiments by the Genotype-Tissue Expression (GTEx) project, to create a new catalog of human genes and transcripts, called CHESS. The new database contains 42,611 genes, of which 20,352 are potentially protein-coding and 22,259 are noncoding, and a total of 323,258 transcripts. These include 224 novel protein-coding genes and 116,156 novel transcripts. We detected over 30 million additional transcripts at more than 650,000 genomic loci, nearly all of which are likely nonfunctional, revealing a heretofore unappreciated amount of transcriptional noise in human cells. The CHESS database is available at http://ccb.jhu.edu/chess. The online version of this article (10.1186/s13059-018-1590-2) contains supplementary material, which is available to authorized users.
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通讯作者：
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