Full-range magnetic manipulation of droplets via surface energy traps enables complex bioassays.
Full-range magnetic manipulation of droplets via surface energy traps enables complex bioassays.
复制标题
DOI：
10.1002/adma.201300383
复制
发表时间：
2013-06-04
影响因子：
29.4
通讯作者：
Wang, Tza-Huei
中科院分区：
文献类型：
--
作者：
Zhang, Yi;Wang, Tza-Huei
在完全集成和可扩展的芯片实验室（Lab-on-a-chip，简称LAB）设备上实施复杂的生物分析测定在关键的生物医学应用中具有巨大的潜力，如即时诊断和高通量筛选[1-5]。然而，存在显著的挑战，因为当前的微流控装置主要依赖于连续流微流体，并且需要多方面的流体架构、诸如泵和阀的组件以及外部流体接口来进行复杂的生物测定。为了解决这些基于通道的连续流动系统的挑战，人们对开发基于液滴的微流体系统越来越感兴趣[6-9]。不同的机制已用于液滴致动，包括电润湿[10-14]、磁力[15-18]、光致动[19-21]、表面声波[22]和介电泳[23]。其中，电润湿是最广泛使用的，因为它能够进行全面的流体操作，包括分配，分裂和运输。尽管如此，通过电润湿进行的这种宽范围的流体操作需要封闭或双板配置，其中液滴紧紧夹在用电极图案化的两个基板之间，导致有限的操作液体体积（100 nl-1 μl）[6，8]。由于统计采样误差，这种小的测定体积对于需要大样品体积的测定（例如基于PCR的传染性病原体检测）可能是不切实际的。此外，单独的电润湿仅限于液体处理，并且不能用于操纵在非均相测定中使用的固体材料。通常需要次级机制，如磁力或介电泳，用于颗粒处理[14，24-28]。
Implementing complex bioanalytical assays on fully integrated and scalable lab-on-a-chip (LOC) devices has great potential in key biomedical applications such as point-of-care diagnostics and high-throughput screening [1–5]. However, there are significant challenges because the current LOC devices mostly rely on continuous flow microfluidics and require multifaceted fluidic architectures, components such as pumps and valves, and an external fluid interface to carry out complex bioassays. To address the challenges of these channelbased, continuous flow systems, there is increasing interest in developing droplet-based microfluidic systems [6–9]. Diverse mechanisms have been used for droplet actuation, including electrowetting [10–14], magnetic force [15–18], photo-actuation [19–21], surface acoustic wave [22], and dielectrophoresis [23]. Of these, electrowetting is most widely used because it is capable of comprehensive fluidic operation including dispensing, splitting, and transport. Nonetheless, such a wide range of fluidic operation by electrowetting requires a closed or two-plate configuration, in which droplets are tightly sandwiched between two substrates patterned with electrodes, resulting in a restricted operating liquid volume (100s nl-1 μl)[6, 8]. This small assay volume may be impractical for assays that require large sample volume, such as PCR-based detection of infectious agents due to statistical sampling errors. Furthermore, electrowetting alone is limited to liquid handling, and cannot be used to manipulate the solid materials used in heterogeneous assays. Usually a secondary mechanism, such as magnetic forces or dielectrophoresis, is needed for particle handling [14, 24–28].
登录
查看更多内容
影响因子：
2.7
作者：
Cho, SK;Moon, HJ;Kim, CJ
通讯作者：
Kim, CJ
影响因子：
2.1
作者：
CHO, CC;WALLACE, RM;FILESSESLER, LA
通讯作者：
FILESSESLER, LA
影响因子：
16.6
作者：
Pipper, Juergen;Zhang, Yi;Hsieh, Tseng-Ming
通讯作者：
Hsieh, Tseng-Ming
影响因子：
8.4
作者：
Shikida, M;Takayanagi, K;Sato, K
通讯作者：
Sato, K
影响因子：
6.1
作者：
Guttenberg, Z;Müller, H;Wixforth, A
通讯作者：
Wixforth, A