Serine Hydroxymethyltransferase 1 Is Essential for Primary-Root Growth at Low-Sucrose Conditions.
Serine Hydroxymethyltransferase 1 Is Essential for Primary-Root Growth at Low-Sucrose Conditions.
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DOI：
10.3390/ijms23094540
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发表时间：
2022-04-20
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
植物根是从土壤或介质中吸收养分的重要器官。蔗糖作为根发育中重要的碳源，蔗糖饥饿会干扰植物细胞的氧化还原状态。然而，蔗糖饥饿时根系生长的机制仍不清楚。在这里，我们报道 SHMT1（丝氨酸羟甲基转移酶 1）在初生根生长中发挥着至关重要的作用。在无蔗糖条件下，SHMT1 突变导致糖水平降低、H2O2 积累过多和严重的根生长停滞，而 SHMT1 过表达的植物糖分增加、H2O2 水平降低，且初生根更长。蔗糖供应完全恢复了shm1-2的根部生长，但单独使用CO2则不能，并且在蔗糖条件下SHMT1在根部比芽中稳定得多，这表明SHMT1在根部的积累对于蔗糖积累和根部生长至关重要。通过应用 GSH 进一步清除 ROS，或通过应用夹竹桃麻素或 RBOHD 突变抑制 ROS 合成，降低了 H2O2 水平，并部分恢复了 shm1-2 在低糖条件下的根生长停滞表型，表明 SHMT1 通过蔗糖介导的 ROS 积累来调节根生长。我们的研究结果证明了 SHMT1 通过调节根中蔗糖积累和 ROS 稳态在初生根生长中的作用。
Plant roots are essential organs for absorbing nutrients from the soil or medium. Sucrose functions as a vital carbon source in root development, and sucrose starvation interferes with the redox state of plant cells. However, the mechanism of root growth at sucrose starvation remains unclear. Here, we report that SHMT1 (serine hydroxymethyltransferase 1) plays a crucial role in primary-root growth. SHMT1 mutation caused decreased sugar levels, excessive H2O2 accumulation, and severe root-growth arrest at sucrose-free conditions, whereas plants with SHMT1 overexpression had increased sugar and decreased H2O2 levels, and longer primary roots. Sucrose supply fully restored root growth of shm1-2, but CO2 alone could not, and SHMT1 is much more stable in roots than shoots at sucrose conditions, suggesting that SHMT1 accumulation in roots is critical for sucrose accumulation and root growth. Further ROS scavenging by GSH application or ROS synthesis inhibition by apocynin application or RBOHD mutation reduced H2O2 levels and partially restored the root-growth arrest phenotype of shm1-2 at low-sucrose conditions, suggesting that SHMT1 modulates root growth via sucrose-mediated ROS accumulation. Our findings demonstrated the role of SHMT1 in primary-root growth by regulating sucrose accumulation and ROS homeostasis in roots.
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