Extracellular ATP is involved in regulating Arabidopsis seed germination
Extracellular ATP is involved in regulating Arabidopsis seed germination
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细胞外 ATP 参与调节拟南芥种子萌发
DOI：
10.1007/s00425-022-03839-w
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发表时间：
2022-02
期刊：
影响因子：
4.3
通讯作者：
Hanqing Feng
中科院分区：
文献类型：
--
作者：
Hanqi Wang;Yuejing Zhang;Zhenzhen Shi;Hailong Pang;Lingyun Jia;Hanqing Feng
胞外ATP可通过其受体DORN1对种子萌发起负性调节作用。摘要胞外ATP(ExATP)是调节植物生长发育和对外界环境反应的信号分子。在本研究中，我们研究了exATP可能参与了拟南芥种子萌发的调控。用外源三磷酸腺苷处理拟南芥种子可以延缓种子萌发，提示外源三磷酸腺苷可能是种子萌发的抑制因子。在拟南芥种子萌发过程中，种子的exATP水平呈现一过性升高。当外源葡萄糖-己糖激酶体系有效降低萌发过程中拟南芥种子的exATP水平时，萌发率显著提高，而ATP水解物对萌发无明显影响。进一步的研究表明，与野生型(WT)植物的种子相比，dorn1-3突变植株(exATP受体突变)的种子具有更高的发芽率。此外，Dorn1-3突变体种子对外源ATP对种子萌发的延迟效应的敏感性低于WT种子。与WT种子相比，Dorn1-3突变体种子在吸胀前具有较高的GA(赤霉素)含量、较低的ABA(脱落酸)含量和较低的ABA/GA比值。外源三磷酸腺苷对种子萌发的调控依赖于外界温度。这些数据表明exATP参与了对拟南芥种子萌发的调控。
Main conclusionExtracellular ATP level induced a transient increase during germination of Arabidopsis seeds, and extracellular ATP could negatively regulate the seed germination by its receptor, DORN1.AbstractExtracellular ATP (exATP) acts as a signal molecule for regulating growth, development, and responses of plants to external environments. In this study, we investigated the possible involvement of exATP in regulating the seed germination ofArabidopsis thaliana. Treatments of Arabidopsis seeds with exogenous ATP delayed seed germination, suggesting that exATP could be a repressor for seed germination. During the germination of Arabidopsis seeds, the exATP level of the seeds presented a transient increase. When exogenous application of the glucose-hexokinase system effectively decreased the exATP level of the Arabidopsis seeds during germination, the percentage of germination was significantly enhanced, while the products of ATP hydrolysis had no effects on the germination. Further studies showed that the seeds ofdorn1–3 mutant plants (mutation in exATP receptor) showed a higher germination percentage, compared to the seeds of wide type (WT) plants. In addition, thedorn1–3 mutant seeds were less sensitive to the delay-effect of exogenous ATP on seed germination than the WT seeds. Thedorn1–3 mutant seeds presented a higher GA (gibberellin) content, lower ABA (abscisic acid) content, and lower ratio of ABA/GA contents before the imbibition, compared to the WT seeds. The regulation of seed germination by exATP was dependent on the external temperature. These data suggest that exATP is involved in regulating Arabidopsis seed germination.
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影响因子：
7.4
作者：
Tanaka, Kiwamu;Swanson, Sarah J.;Stacey, Gary
通讯作者：
Stacey, Gary
DOI：
10.5564/mjas.v26i01.1200
发表时间：
2019-04
期刊：
Mongolian Journal of Agricultural Sciences
影响因子：
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作者：
J Bayarmaa-;D Purev-
通讯作者：
J Bayarmaa-;D Purev-
拟南芥质膜定位 ATP 转运蛋白对花药发育重要的鉴定[W]
影响因子：
11.6
作者：
Rieder B;Neuhaus HE
通讯作者：
Neuhaus HE
影响因子：
7.4
作者：
Clark, Greg;Torres, Jonathan;Roux, Stanley J.
通讯作者：
Roux, Stanley J.
影响因子：
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作者：
Sawsan Mohammed;M. Qoronfleh
通讯作者：
Sawsan Mohammed;M. Qoronfleh