Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats
Lycium Barbarum Polysaccharides Improve Retinopathy in Diabetic Sprague-Dawley Rats
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枸杞多糖改善糖尿病 Sprague-Dawley 大鼠的视网膜病变
DOI：
10.1155/2018/7943212
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发表时间：
2018-11
影响因子：
--
通讯作者：
Andy Li
中科院分区：
文献类型：
--
作者：
姚青;杨怡;芦晓红;张茜;罗明秀;潘燕;Andy Li
糖尿病视网膜病变（diabetic retinopathy，DR）已成为发达国家劳动年龄人群中最常见的致盲原因。本研究以糖尿病大鼠为研究对象，采用枸杞多糖（LBP）400 mg/kg/d或200 mg/kg/d灌胃治疗糖尿病大鼠20周。采用视网膜电图（ERG）和激光多普勒血流检测评价视网膜功能，常规组织学和超微结构检查评价视网膜形态学改变，免疫组化、Western blotting和RT-PCR检测促血管生成因子和抗血管生成因子的蛋白和mRNA水平。结果表明，糖尿病可使视网膜电图a波、b波和振荡电位的振幅降低，视网膜血流量减少，视网膜厚度减少，视网膜毛细血管基底膜厚度增加。此外，糖尿病增加了促血管生成GFAP和VEGF的mRNA和蛋白表达，抑制了抗血管生成PEDG的水平。LBP治疗完全或部分逆转了糖尿病引起的改变。它的结论是，LBP保护糖尿病大鼠的视网膜功能和形态，可能是通过重新安装的促血管生成和抗血管生成因子之间的平衡，从而减少新生血管。枸杞多糖可作为DR的治疗药物。
Diabetic retinopathy (DR) has become the most frequent cause of impaired visual acuity and blindness in working-age population in developed countries. Here we use diabetic rats to clarify the role of Lycium barbarum polysaccharides (LBP) on DR. We treated diabetic rats with LBP (400 mg/kg/d or 200 mg/kg/d) orally for 20 weeks. Electroretinogram (ERGs) and Laser Doppler blood flow were measured to assess the retinal function, routine histology and ultrastructural studies were performed to evaluate the morphological alterations, and immunohistochemistry, western blotting, and RT-PCR were conducted to detect the protein and mRNA levels of pro- and antiangiogenic factors. The results showed that diabetes suppressed the amplitudes of a-wave, b-wave, and oscillatory potential in ERG, reduced retinal blood flow, decreased the thickness of the retina, and increased the thickness of basement membrane of the retinal capillary. Furthermore, diabetes increased the mRNA and protein expressions of proangiogenic GFAP and VEGF and suppressed the levels of antiangiogenic PEDG. Treatment with LBP either completely or partially reversed the alterations caused by diabetes. It is concluded that the LBP protects retinal function and morphology in diabetic rats, probably through reinstallation of the balance between proangiogenic and antiangiogenic factors, which reduces neovascularization. LBP could be used as a therapeutic drug for DR.
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影响因子：
81.5
作者：
T. Wong;C. Cheung;M. Larsen;Sanjay Sharma;R. Simó
通讯作者：
T. Wong;C. Cheung;M. Larsen;Sanjay Sharma;R. Simó
DOI：
10.1155/2014/326107
发表时间：
2014
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Zareisedehizadeh S;Tan CH;Koh HL
通讯作者：
Koh HL
影响因子：
15.9
作者：
Barber, AJ;Lieth, E;Gardner, TW
通讯作者：
Gardner, TW
影响因子：
7.7
作者：
Lieth, E;Barber, AJ;Strother, JM
通讯作者：
Strother, JM
影响因子：
5
作者：
MUNK, PL;VELLET, AD;COLLYER, RT
通讯作者：
COLLYER, RT