CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew.
CRISPR/Cas9-Mediated Generation of Pathogen-Resistant Tomato against Tomato Yellow Leaf Curl Virus and Powdery Mildew.
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DOI：
10.3390/ijms22041878
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发表时间：
2021-02-13
影响因子：
5.6
通讯作者：
Kim JY
中科院分区：
文献类型：
--
作者：
Pramanik D;Shelake RM;Park J;Kim MJ;Hwang I;Park Y;Kim JY
番茄是世界上主要的蔬菜作物之一。番茄黄化曲叶病毒（TYLCV）和粉孢菌属（Oidium sp.）是引起番茄黄化曲叶病和白粉病的毁灭性病原。这些病毒和真菌病原体降低番茄作物产量，每年造成巨大的经济损失。几个商业番茄品种包括分别携带TYLCV和白粉病的易感性（S-基因）因子的Ty-5（SlPelo）和抗白粉病基因座o 1（SlMlo 1）基因座。成簇规则间隔短回文重复序列（CRISPR）/CRISPR相关蛋白（Cas）是开发抗病作物品种的有价值的基因组编辑工具。在这方面，靶向由宿主植物基因组而不是病毒基因组编码的易感性因子是一种很有前途的方法，可以在不需要CRISPR组分稳定遗传的情况下实现病原体抗性。在这项研究中，CRISPR/Cas9系统被用于靶向优良番茄栽培品种BN-86中的SlPelo和SlMlo 1用于性状渐渗，以赋予宿主介导的针对病原体的免疫力。成功产生携带双等位基因插入缺失突变的SlPelo敲除系。在SlPelo突变株系中的病原体抗性测定证实了TYLCV的抑制积累并限制了向未接种植物部分的传播。所产生的SlMlol的敲除系显示出对白粉病真菌的完全抗性。总的来说，我们的结果证明了CRISPR/Cas9系统在引入靶向诱变以快速开发番茄中的病原体抗性品种方面的效率。
Tomato is one of the major vegetable crops consumed worldwide. Tomato yellow leaf curl virus (TYLCV) and fungal Oidium sp. are devastating pathogens causing yellow leaf curl disease and powdery mildew. Such viral and fungal pathogens reduce tomato crop yields and cause substantial economic losses every year. Several commercial tomato varieties include Ty-5 (SlPelo) and Mildew resistance locus o 1 (SlMlo1) locus that carries the susceptibility (S-gene) factors for TYLCV and powdery mildew, respectively. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) is a valuable genome editing tool to develop disease-resistant crop varieties. In this regard, targeting susceptibility factors encoded by the host plant genome instead of the viral genome is a promising approach to achieve pathogen resistance without the need for stable inheritance of CRISPR components. In this study, the CRISPR/Cas9 system was employed to target the SlPelo and SlMlo1 for trait introgression in elite tomato cultivar BN-86 to confer host-mediated immunity against pathogens. SlPelo-knockout lines were successfully generated, carrying the biallelic indel mutations. The pathogen resistance assays in SlPelo mutant lines confirmed the suppressed accumulation of TYLCV and restricted the spread to non-inoculated plant parts. Generated knockout lines for the SlMlo1 showed complete resistance to powdery mildew fungus. Overall, our results demonstrate the efficiency of the CRISPR/Cas9 system to introduce targeted mutagenesis for the rapid development of pathogen-resistant varieties in tomato.
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DOI：
10.21273/jashs.123.6.1004
发表时间：
1998-11-01
影响因子：
1.9
作者：
Friedmann, M;Lapidot, M;Pilowsky, M
通讯作者：
Pilowsky, M
影响因子：
56.9
作者：
Jinek, Martin;Chylinski, Krzysztof;Charpentier, Emmanuelle
通讯作者：
Charpentier, Emmanuelle
影响因子：
2.7
作者：
Kil, Eui-Joon;Byun, Hee-Seong;Lee, Sukchan
通讯作者：
Lee, Sukchan
影响因子：
5.1
作者：
Cui, Xiucheng;Balcerzak, Margaret;Ouellet, Therese
通讯作者：
Ouellet, Therese
影响因子：
4.5
作者：
Lapidot M;Karniel U;Gelbart D;Fogel D;Evenor D;Kutsher Y;Makhbash Z;Nahon S;Shlomo H;Chen L;Reuveni M;Levin I
通讯作者：
Levin I