Enhanced microarray performance using low complexity representations of the transcriptome.
Enhanced microarray performance using low complexity representations of the transcriptome.
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DOI：
10.1093/nar/gni095
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发表时间：
2005-06-24
影响因子：
14.9
通讯作者：
Welsh J
中科院分区：
文献类型：
--
作者：
Rondeau G;McClelland M;Nguyen T;Risques R;Wang Y;Judex M;Cho AH;Welsh J
由于浓度对杂交动力学和信噪比的影响，使用微阵列检测低丰度mrna比检测高丰度mrna更困难。本报告描述了使用mRNA的低复杂性表示（lcr）作为cDNA微阵列的目标。lcr中的单个序列比其衍生的mRNA群体中具有更高的代表性，从而导致有利的杂交动力学。LCR靶标允许测量丰度变化，这是使用寡核苷酸（dT）引发靶合成难以测量的。在一项实验中，血清饥饿成纤维细胞对重新引入血清有反应，一个oligo（dT）引物靶标和三个lcr检测到的差异调节基因数量是单独用oligo（dT）引物靶标检测到的两倍。因此，这种目标制备策略大大提高了cDNA微阵列的灵敏度。
Low abundance mRNAs are more difficult to examine using microarrays than high abundance mRNAs due to the effect of concentration on hybridization kinetics and signal-to-noise ratios. This report describes the use of low complexity representations (LCRs) of mRNA as the targets for cDNA microarrays. Individual sequences in LCRs are more highly represented than in the mRNA populations from which they are derived, leading to favorable hybridization kinetics. LCR targets permit the measurement of abundance changes that are difficult to measure using oligo(dT) priming for target synthesis. An oligo(dT)-primed target and three LCRs detect twice as many differentially regulated genes as could be detected by the oligo(dT)-primed target alone, in an experiment in which serum-starved fibroblasts responded to the reintroduction of serum. Thus, this target preparation strategy considerably increases the sensitivity of cDNA microarrays.
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影响因子：
30.8
作者：
Armstrong, SA;Staunton, JE;Korsmeyer, SJ
通讯作者：
Korsmeyer, SJ
影响因子：
56.9
作者：
Golub, TR;Slonim, DK;Lander, ES
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Lander, ES
影响因子：
56.9
作者：
LIANG, P;PARDEE, AB
通讯作者：
PARDEE, AB
影响因子：
14.9
作者：
MCCLELLAND, M;RALPH, D;WELSH, J
通讯作者：
WELSH, J
影响因子：
14.9
作者：
WELSH, J;CHADA, K;MCCLELLAND, M
通讯作者：
MCCLELLAND, M