The pip1s Quintuple Mutants Demonstrate the Essential Roles of PIP1s in the Plant Growth and Development of Arabidopsis.
The pip1s Quintuple Mutants Demonstrate the Essential Roles of PIP1s in the Plant Growth and Development of Arabidopsis.
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pip1s五重突变体展示了PIP1s在拟南芥植物生长和发育中的重要作用
DOI：
10.3390/ijms22041669
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发表时间：
2021-02-07
影响因子：
5.6
通讯作者：
Zhang G
中科院分区：
文献类型：
--
作者：
Wang X;Wu Y;Liu Z;Liu T;Zheng L;Zhang G
质膜内源蛋白(PIP)通过质膜运输水、二氧化碳和小的中性溶质。在这项研究中，我们使用聚集性规则间隔短回文重复序列和CRISPR相关蛋白9系统(CRISPR/CAS9)来突变PIP1；4和PIP1；5在PIP1；1，2，3三重突变体中产生PIP1；1，2，3，4，5(PIP1；1，2，3，4，5(PIP1−)五重突变。与野生型植株相比，−突变体具有较小的莲座叶和花朵、较少的莲座叶数、较多的未发育角果、较短的角果和较少的种子。−突变体的花粉萌发率显著低于野生型，且突变体−的花粉外壁发生变形。转录组分析表明，−突变体中许多参与叶片、花和花粉发育的关键基因和转录因子的表达发生了显著变化，这表明突变体不仅直接影响植物的水力和固碳，而且还调节相关基因的表达，从而影响植物的生长发育。
Plasma membrane intrinsic proteins (PIPs) transport water, CO2 and small neutral solutes across the plasma membranes. In this study, we used the clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 system (CRISPR/Cas9) to mutate PIP1;4 and PIP1;5 in a pip1;1,2,3 triple mutant to generate a pip1;1,2,3,4,5 (pip1s−) quintuple mutant. Compared to the wild-type (WT) plant, the pip1s− mutants had smaller sized rosette leaves and flowers, less rosette leaf number, more undeveloped siliques, shorter silique and less seeds. The pollen germination rate of the pip1s− mutant was significantly lower than that of the WT and the outer wall of the pip1s− mutant’s pollen was deformed. The transcriptomic analysis showed significant alterations in the expression of many key genes and transcription factors (TFs) in the pip1s− mutant which involved in the development of leaf, flower and pollen, suggesting that the mutant of PIP1s not only directly affects hydraulics and carbon fixation, but also regulates the expression of related genes to affect plant growth and development.
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