Cross-species prophylactic efficacy of Sm-p80-based vaccine and intracellular localization of Sm-p80/Sm-p80 ortholog proteins during development in Schistosoma mansoni, Schistosoma japonicum, and Schistosoma haematobium.
Cross-species prophylactic efficacy of Sm-p80-based vaccine and intracellular localization of Sm-p80/Sm-p80 ortholog proteins during development in Schistosoma mansoni, Schistosoma japonicum, and Schistosoma haematobium.
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DOI：
10.1007/s00436-017-5634-4
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发表时间：
2017-11
影响因子：
2
通讯作者：
Siddiqui AA
中科院分区：
文献类型：
--
作者：
Molehin AJ;Sennoune SR;Zhang W;Rojo JU;Siddiqui AJ;Herrera KA;Johnson L;Sudduth J;May J;Siddiqui AA
血吸虫病仍然是一个重大的全球健康问题。尽管开展了大规模的血吸虫病控制工作，但仍存在明显的局限性，如可能出现的耐药性和再感染率，突出表明需要有效的血吸虫病疫苗。基于曼氏血吸虫钙蛋白酶大亚基（Sm-p80）的疫苗制剂在预防曼氏血吸虫感染方面显示出显著的功效。曼索尼挑战小鼠和狒狒的感染。在本研究中，我们评估了Sm-p80疫苗对S. japonicum和S.在啮齿动物模型中的嗜血杆菌攻击感染。我们还阐明了Sm-p80和Sm-p80同源蛋白在不同发育阶段的表达。mansoni，S. haematobium和S.山茱萸Sm-p80疫苗免疫后，减虫率为46.75%。日本血吸虫攻击感染小鼠。DNA初免/蛋白加强（1+1剂量，一天给药）导致S.嗜血杆菌-仓鼠感染/攻击模型。疫苗接种后，在两种链球菌中观察到平衡的Th 1（IFN-γ、TNF-α、IL-2和IL-12）和Th 2（IL-4、IgG 1）类型反应。japonicum和S.这些试验与Sm-p80疫苗的预防效力有关。免疫组化结果表明，Sm-p80/Sm-p80直系同源蛋白表达在不同的生命周期阶段的三个主要的人类物种的染色体研究。本研究中提供的数据加强了Sm-p80疫苗在世界不同地理区域发生的肝/肠和泌尿生殖道血吸虫病中的潜力。Sm-p80/Sm-p80蛋白同源物在不同生命周期中的差异表达使得该疫苗在针对不同水平的感染、疾病和传播方面具有潜在的用途。
Schistosomiasis remains a major global health problem. Despite large-scale schistosomiasis control efforts, clear limitations such as possible emergence of drug resistance and re-infection rates, highlight the need for an effective schistosomiasis vaccine. Schistosoma mansoni large subunit of calpain (Sm-p80)-based vaccine formulations have shown remarkable efficacy in protecting against S. mansoni challenge infections in mice and baboons. In this study, we evaluated the cross-species protective efficacy of Sm-p80 vaccine against S. japonicum and S. haematobium challenge infections in rodent models. We also elucidated the expression of Sm-p80 and Sm-p80 ortholog proteins in different developmental stages of S. mansoni, S. haematobium and S. japonicum. Immunization with Sm-p80 vaccine reduced worm burden by 46.75% against S. japonicum challenge infection in mice. DNA-prime/protein boost (1+1 dose administered on a single day) resulted in 26.95% reduction in worm burden in S. haematobium-hamster infection/challenge model. A balanced Th1 (IFN-γ, TNF-α, IL-2 and IL-12) and Th2 (IL-4, IgG1) type of responses were observed following vaccination in both S. japonicum and S. haematobium challenge trials and these are associated with the prophylactic efficacy of Sm-p80 vaccine. Immunohistochemistry demonstrated that Sm-p80/Sm-p80 ortholog proteins are expressed in different life cycle stages of the three major human species of schistosomes studied. The data presented in this study reinforce the potential of Sm-p80-based vaccine for both hepatic/intestinal and urogenital schistosomiasis occurring in different geographical areas of the world. Differential expression of Sm-p80/Sm-p80 protein orthologs in different life cycle makes this vaccine potentially useful in targeting different levels of infection, disease and transmission.
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影响因子：
3.8
作者：
Hotez PJ;Alvarado M;Basáñez MG;Bolliger I;Bourne R;Boussinesq M;Brooker SJ;Brown AS;Buckle G;Budke CM;Carabin H;Coffeng LE;Fèvre EM;Fürst T;Halasa YA;Jasrasaria R;Johns NE;Keiser J;King CH;Lozano R;Murdoch ME;O'Hanlon S;Pion SD;Pullan RL;Ramaiah KD;Roberts T;Shepard DS;Smith JL;Stolk WA;Undurraga EA;Utzinger J;Wang M;Murray CJ;Naghavi M
通讯作者：
Naghavi M
影响因子：
11.1
作者：
Angeles JM;Leonardo LR;Goto Y;Kirinoki M;Villacorte EA;Hakimi H;Moendeg KJ;Lee S;Rivera PT;Inoue N;Chigusa Y;Kawazu S
通讯作者：
Kawazu S
影响因子：
2.2
作者：
de Melo, T. T.;de Sena, I. C.;Fonseca, C. T.
通讯作者：
Fonseca, C. T.
影响因子：
5.5
作者：
Ahmad G;Zhang W;Torben W;Damian RT;Wolf RF;White GL;Chavez-Suarez M;Kennedy RC;Siddiqui AA
通讯作者：
Siddiqui AA
DOI：
10.1080/00034983.1978.11719309
发表时间：
1978-01-01
影响因子：
--
作者：
GHANDOUR, AM
通讯作者：
GHANDOUR, AM