Development of a method for the purification and culture of rodent astrocytes.
Development of a method for the purification and culture of rodent astrocytes.
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DOI：
10.1016/j.neuron.2011.07.022
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发表时间：
2011-09-08
期刊：
影响因子：
16.2
通讯作者：
Barres BA
中科院分区：
文献类型：
--
作者：
Foo LC;Allen NJ;Bushong EA;Ventura PB;Chung WS;Zhou L;Cahoy JD;Daneman R;Zong H;Ellisman MH;Barres BA
长期以来，无法纯化和培养星形胶质细胞一直阻碍着对其功能的研究。虽然星形胶质细胞的前体细胞可以从新生儿脑中培养出来，但从出生后的脑中培养成熟的星形胶质细胞还不可能。在这里，我们报告了一种新的免疫泛素纯化星形胶质细胞的方法。这些星形胶质细胞在培养过程中经历了凋亡，但血管细胞和HBEGF促进了它们在无血清培养中的存活。我们发现，一些发育中的星形胶质细胞在体内通常会发生凋亡，并且绝大多数星形胶质细胞接触血管，这表明星形胶质细胞与血管匹配是通过竞争血管衍生的营养因子，如HBEGF。与传统的星形胶质细胞培养相比，培养的纯化后星形胶质细胞的基因图谱更接近体内星形胶质细胞。虽然这些星形胶质细胞强烈促进突触的形成和功能，但它们不会对刺激做出反应而分泌谷氨酸。
The inability to purify and culture astrocytes has long hindered studies of their function. Whereas astrocyte progenitor cells can be cultured from neonatal brain, culture of mature astrocytes from postnatal brain has not been possible. Here we report a new method to prospectively purify astrocytes by immunopanning. These astrocytes undergo apoptosis in culture, but vascular cells and HBEGF promote their survival in serum-free culture. We found that some developing astrocytes normally undergo apoptosis in vivo and that the vast majority of astrocytes contact blood vessels, suggesting that astrocytes are matched to blood vessels by competing for vascular-derived trophic factors such as HBEGF. Compared to traditional astrocyte cultures, the gene profiles of the cultured purified postnatal astrocytes much more closely resemble those of in vivo astrocytes. Although these astrocytes strongly promote synapse formation and function, they do not secrete glutamate in response to stimulation.
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DOI：
10.1083/jcb.85.3.890
发表时间：
1980-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
McCarthy KD;de Vellis J
通讯作者：
de Vellis J
影响因子：
4.8
作者：
Gan, Hui K.;Walker, Francesca;Johns, Terrance G.
通讯作者：
Johns, Terrance G.
影响因子：
3.3
作者：
Hildebrand, B;Olenik, C;Meyer, DK
通讯作者：
Meyer, DK
通过神经胶质调节突触连通性。
影响因子：
64.8
作者：
Eroglu, Cagla;Barres, Ben A.
通讯作者：
Barres, Ben A.
影响因子：
3.7
作者：
Daneman R;Zhou L;Agalliu D;Cahoy JD;Kaushal A;Barres BA
通讯作者：
Barres BA