Antibiofilm Efficacy of Luteolin Against Single and Dual Species of Candida albicans and Enterococcus faecalis.
Antibiofilm Efficacy of Luteolin Against Single and Dual Species of Candida albicans and Enterococcus faecalis.
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DOI：
10.3389/fmicb.2021.715156
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发表时间：
2021
影响因子：
5.2
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
Fu Y;Wang W;Zeng Q;Wang T;Qian W
白色念珠菌和粪肠球菌生物膜相关感染一直是医学界面临的巨大挑战。然而，天然产物对抗白色念珠菌和粪肠球菌混合生物膜的功效仍未得到充分利用。本研究的目的是评估木犀草素对体外单一和混合培养物中白色念珠菌和粪肠球菌的浮游细胞生长、粘附和生物膜形成的功效。结果表明，木犀草素对白色念珠菌、粪肠球菌和混合培养物的浮游细胞的最低抑制浓度分别为32和64 μg ml-1。结果表明，主要通过结晶紫染色法（CVSA）、光学显微镜、场发射扫描电子显微镜（FESEM）和共聚焦激光扫描显微镜（CLSM）证实，在木犀草素存在下，白色念珠菌和粪肠球菌单种和双种生物膜形成的单种和双种生物膜的生物膜生物量、活力、结构和组成存在显着变化。 CLSM发现经木犀草素处理的单种和双种生物膜对抗生素的耐受性明显降低，并且生物膜基质成分（主要是多糖和蛋白质）损失。此外，木犀草素可有效灭活生物膜细胞，并通过 CVSA、FESEM 和 CLSM 破坏单物种和双物种预先形成的生物膜结构。总的来说，这些数据表明木犀草素作为一种有前途的抗生物膜剂的潜力，用于治疗由单一和双种白色念珠菌和粪肠球菌引起的生物膜相关感染。
Candida albicans and Enterococcus faecalis biofilm-associated infections have been a huge challenge to the medical community. However, the efficacy of natural products against mixed biofilms of C. albicans and E. faecalis still remains largely unexploited. The aim of this study was to evaluate the efficacy of luteolin against planktonic cell growth, adhesion, and biofilm formation of C. albicans and E. faecalis in single and mixed cultures in vitro. The results showed that the minimum inhibitory concentrations of luteolin against planktonic cells of C. albicans, E. faecalis, and mixed cultures were 32 and 64 μg ml–1, respectively. The results displayed that a remarkable variation in biofilm biomass, viability, structure, and composition of single and dual-species biofilms formed by mono- and dual-species biofilms of C. albicans and E. faecalis in the presence of luteolin was confirmed by mainly crystal violet staining assay (CVSA), optical microscope, field emission scanning electron microscope (FESEM), and confocal laser scanning microscope (CLSM). The tolerance of luteolin-treated single- and dual-species biofilms to antibiotics was found to obviously decrease, and the loss of biofilm matrix components (mainly polysaccharides and proteins) was revealed by CLSM. Moreover, luteolin was effective at inactivating biofilm cells, as well as destructing preformed biofilm structures by single and dual species by CVSA, FESEM, and CLSM. Collectively, these data indicate the potential of luteolin as a promising antibiofilm agent for the therapeutic management of biofilm-related infections induced by single and dual species of C. albicans and E. faecalis.
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