Major Histocompatibility Complex I and II Expression and Lymphocytic Subtypes in Muscle of Horses with Immune-Mediated Myositis.
Major Histocompatibility Complex I and II Expression and Lymphocytic Subtypes in Muscle of Horses with Immune-Mediated Myositis.
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DOI：
10.1111/jvim.14371
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发表时间：
2016-07
影响因子：
2.6
通讯作者：
Valberg SJ
中科院分区：
文献类型：
--
作者：
Durward-Akhurst SA;Finno CJ;Barnes N;Shivers J;Guo LT;Shelton GD;Valberg SJ
主要组织相容性复合体（MHC）I和II的表达通常不会在肌膜上检测到，但在人和犬的免疫介导的肌炎（IMM）和dysferlin缺陷型肌营养不良症中的淋巴细胞浸润中检测到。确定马的活动性IMM中是否表达肌膜MHC，MHC表达是否与淋巴细胞亚型相关，以及IMM中是否表达dysferlin。21匹季马相关品种的IMM马，3匹健康马和6匹疾病对照马（3匹牧场肌病，3匹淀粉酶抗性多糖沉积性肌病[PSSM]）。对IMM和对照马的存档肌肉进行MHC I、II和CD 4+、CD 8+、CD 20+淋巴细胞的免疫组织化学染色。对MHC I、II和淋巴细胞亚型进行评分。对dysferlin、dystrophin和α-肌聚糖进行免疫荧光染色。肌膜MHC I和II的表达分别在17/21和15/21的IMM马，并在特定的纤维PSSM马，但不是健康或牧场肌病对照。20/21匹IMM马的肌肉中存在CD 4+、CD 8+和CD 20+细胞，其中10/21匹IMM马以CD 4+为主，6/21匹IMM马以CD 8+为主。在IMM和对照肌肉中，Dysferlin、肌养蛋白和α-肌聚糖染色相似。dysferlin、dystrophin和a-肌聚糖的缺陷与IMM无关。肌膜MHC I和II在IMM马的肌纤维中的比例表达以及淋巴细胞浸润支持IMM的免疫介导的病因学。在没有淋巴细胞浸润的情况下，MHC表达也发生在PSSM马的特定肌纤维中。
Major histocompatibility complex (MHC) I and II expression is not normally detected on sarcolemma, but is detected with lymphocytic infiltrates in immune‐mediated myositis (IMM) of humans and dogs and in dysferlin‐deficient muscular dystrophy. To determine if sarcolemmal MHC is expressed in active IMM in horses, if MHC expression is associated with lymphocytic subtype, and if dysferlin is expressed in IMM. Twenty‐one IMM horses of Quarter Horse‐related breeds, 3 healthy and 6 disease controls (3 pasture myopathy, 3 amylase‐resistant polysaccharide storage myopathy [PSSM]). Immunohistochemical staining for MHC I, II, and CD4+, CD8+, CD20+ lymphocytes was performed on archived muscle of IMM and control horses. Scores were given for MHC I, II, and lymphocytic subtypes. Immunofluorescent staining for dysferlin, dystrophin, and a‐sarcoglycan was performed. Sarcolemmal MHC I and II expression was detected in 17/21 and 15/21 of IMM horses, respectively, and in specific fibers of PSSM horses, but not healthy or pasture myopathy controls. The CD4+, CD8+, and CD20+ cells were present in 20/21 IMM muscles with CD4+ predominance in 10/21 and CD8+ predominance in 6/21 of IMM horses. Dysferlin, dystrophin, and a‐sarcoglycan staining were similar in IMM and control muscles. Deficiencies of dysferlin, dystrophin, and a‐sarcoglycan are not associated with IMM. Sarcolemmal MHC I and II expression in a proportion of myofibers of IMM horses in conjunction with lymphocytic infiltration supports an immune‐mediated etiology for IMM. The MHC expression also occured in specific myofibers in PSSM horses in the absence of lymphocytic infiltrates.
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影响因子：
1.8
作者：
Shelton, G. Diane;Hoffman, Eric P.;Nagaraju, Kanneboyina
通讯作者：
Nagaraju, Kanneboyina
影响因子：
2.4
作者：
Morita, T;Shimada, A;Mabuchi, Y
通讯作者：
Mabuchi, Y
影响因子：
2.4
作者：
McCue, M. E.;Armien, A. G.;Valberg, S. J.
通讯作者：
Valberg, S. J.
DOI：
10.1097/00005072-199603000-00009
发表时间：
1996-03-01
影响因子：
3.2
作者：
Tews, DS;Goebel, HH
通讯作者：
Goebel, HH
影响因子：
9.9
作者：
Gallardo, E;Rojas-García, R;Illa, I
通讯作者：
Illa, I