Paraffin Immunofluorescence: A Valuable Ancillary Technique in Renal Pathology.
Paraffin Immunofluorescence: A Valuable Ancillary Technique in Renal Pathology.
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DOI：
10.1016/j.ekir.2018.07.008
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发表时间：
2018-11
影响因子：
6
通讯作者：
Said SM
中科院分区：
文献类型：
--
作者：
Nasr SH;Fidler ME;Said SM
冷冻组织的免疫荧光是评估肾脏免疫沉积的金标准免疫组织化学技术。当没有冷冻组织或缺乏肾小球时，抗原提取后可对石蜡组织进行免疫荧光（石蜡免疫荧光）。石蜡免疫荧光在大多数免疫复合物介导的肾小球肾炎和蛋白异常血症相关的肾脏病变中可获得良好的结果，因此该技术已成为肾脏病理中有价值的抢救技术。此外，新的数据表明，石蜡免疫荧光可以作为一种揭露技术，因为它比冷冻组织免疫荧光在一些肾脏病变（如结晶轻链近端小管病变）中更敏感，并且需要建立某些特殊病变的诊断，如膜样肾小球病伴IgG κ pa沉积和膜增生性肾小球肾炎伴单型Ig沉积。然而，重要的是要认识到与石蜡免疫荧光相关的局限性和缺陷。其中包括C3沉积物检测和原发性膜性肾病诊断的敏感性差。在这里，我们总结了现有的石蜡免疫荧光技术，回顾了它在肾脏病理学中的作用和性能，指出了它的局限性和缺陷，并强调了需要石蜡免疫荧光诊断的肾小球病变的不寻常形式。
Immunofluorescence on frozen tissue is the gold standard immunohistochemical technique for evaluation of immune deposits in the kidney. When frozen tissue is not available or lacks glomeruli, immunofluorescence can be performed on paraffin tissue after antigen retrieval (paraffin immunofluorescence). Excellent results can be obtained by paraffin immunofluorescence in most immune complex–mediated glomerulonephritides and dysproteinemia-associated kidney lesions, and thus this technique has become a valuable salvage technique in renal pathology. Furthermore, new data have emerged suggesting that paraffin immunofluorescence can be used as an unmasking technique, as it is more sensitive than frozen tissue immunofluorescence in some kidney lesions, such as crystalline light chain proximal tubulopathy and is needed to establish the diagnosis of certain unique lesions, such as membranous-like glomerulopathy with masked IgG kappa deposits and membranoproliferative glomerulonephritis with masked monotypic Ig deposits. However, it is important to recognize and be aware of the limitations and pitfalls associated with paraffin immunofluorescence. These include poor sensitivity for detection of C3 deposits and for the diagnosis of primary membranous nephropathy. Here, we summarize the available techniques of paraffin immunofluorescence, review its role and performance as a salvage and unmasking technique in renal pathology, address its limitations and pitfalls, and highlight unusual forms of glomerulopathy that require paraffin immunofluorescence for diagnosis.
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影响因子：
13.2
作者：
Mölne, J;Breimer, ME;Svalander, CT
通讯作者：
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影响因子：
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作者：
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通讯作者：
Khalighi, Mazdak A.
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0.9
作者：
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通讯作者：
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