Detection and characterization of circulating tumor cells in blood of primary breast cancer patients by RT-PCR and comparison to status of bone marrow disseminated cells.
Detection and characterization of circulating tumor cells in blood of primary breast cancer patients by RT-PCR and comparison to status of bone marrow disseminated cells.
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DOI：
10.1186/bcr2349
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Kasimir-Bauer S
中科院分区：
文献类型：
--
作者：
Fehm T;Hoffmann O;Aktas B;Becker S;Solomayer EF;Wallwiener D;Kimmig R;Kasimir-Bauer S
循环肿瘤细胞(CTCs)在原发性乳腺癌患者血液中的作用仍在研究中。我们评估了CTCs在血液中的发生率，评估了CTCs与骨髓中播散性肿瘤细胞(DTC)的相关性，并对CTCs的HER2、雌激素受体(ER)和孕激素受体(PR)的表达进行了表征。采用ADNATEST乳腺癌™(德国ADNAGEN公司)对431例乳腺癌患者外周血中的EPCAM、MUC1和HER2转录本进行分析。ER和PR的表达通过额外的RT-PCR进行评估。使用泛细胞角蛋白抗体A45-B/B3对414例患者的骨髓抽吸物进行了免疫细胞化学分析。414例患者中，107例(24%)检出DTCs，58例(13%)检出CTCs。DTC与原发灶的PR状态相关(P=0.04)，CTC与淋巴结状态(P=0.04)、ER(P=0.05)、PR(P=0.01)显著相关。骨髓中的DTCs与血液中的CTCs呈弱相关(P=0.05)。有趣的是，CTCs的扩散多见于三阴性肿瘤(P=0.01)，而无论原发肿瘤的ER、PR和HER2状态如何，CTCs一般都是三阴性的。(1)由于CTC和DTC之间的一致性较弱，其临床相关性可能不同。(2)原发灶的生物学特性似乎直接影响CTCs的扩散。(3)由于CTC和原发肿瘤的表达谱不同，必须评估其对辅助治疗选择的影响。
The role of circulating tumor cells (CTCs) in blood of primary breast cancer patients is still under investigation. We evaluated the incidence of CTCs in blood, we evaluated the correlation between CTCs and disseminated tumor cells (DTCs) in the bone marrow (BM), and we characterized CTCs for the expression of HER2, the estrogen receptor (ER) and the progesterone receptor (PR). Blood of 431 patients with primary breast cancer were analyzed for EpCAM, MUC1 and HER2 transcripts with the AdnaTest BreastCancer™ (AdnaGen AG, Germany). Expression of the ER and PR was assessed in an additional RT-PCR. BM aspirates from 414 patients were analyzed for DTCs by immunocytochemistry using the pan-cytokeratin antibody A45-B/B3. DTCs were found in 107/414 patients (24%), CTCs were detected in 58/431 (13%) patients. DTCs were associated with PR status of the primary tumor (P = 0.04) and CTCs significantly correlated with nodal status (P = 0.04), ER (P = 0.05), and PR (P = 0.01). DTCs in the BM weakly correlated with CTCs (P = 0.05) in blood. Interestingly, the spread of CTCs was mostly found in triple-negative tumors (P = 0.01) and CTCs in general were mostly found to be triple-negative regardless of the ER, PR and HER2 status of the primary tumor. (1) Due to the weak concordance between CTCs and DTCs the clinical relevance may be different. (2) The biology of the primary tumor seems to direct the spread of CTCs. (3) Since the expression profile between CTCs and the primary tumor differs, the consequence for the selection of adjuvant treatment has to be evaluated.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1186/bcr1783
发表时间：
2007
期刊：
Breast cancer research : BCR
影响因子：
--
作者：
Fehm T;Becker S;Duerr-Stoerzer S;Sotlar K;Mueller V;Wallwiener D;Lane N;Solomayer E;Uhr J
通讯作者：
Uhr J
影响因子：
6.4
作者：
Giatromanolaki, A;Koukourakis, MI;Georgoulias, V
通讯作者：
Georgoulias, V
影响因子：
4.5
作者：
Borgen, E;Naume, B;Pantel, K
通讯作者：
Pantel, K
影响因子：
3.8
作者：
Becker, Sven;Becker-Pergola, Graziella;Fehm, Tanja
通讯作者：
Fehm, Tanja