Mechanism of Isoquercitrin Inducing Apoptosis of HepG2 Cells Through PERK-eIF2a-ATF4 Signaling Pathway
Mechanism of Isoquercitrin Inducing Apoptosis of HepG2 Cells Through PERK-eIF2a-ATF4 Signaling Pathway
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异槲皮苷通过PERK-eIF2a-ATF4信号通路诱导HepG2细胞凋亡的机制
DOI：
10.36347/sasjm.2022.v08i10.010
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发表时间：
2022-10
期刊：
影响因子：
--
通讯作者：
Guihong Huang
中科院分区：
文献类型：
--
作者：
Guojun Jiang;Qingqing Wang;Jiahua Hu;Junhe Huang;Yan Li;Qing Deng;Wenyan Jiang;Guihong Huang
Purpose: To observe the intervention effect of isoquercitrin on related factors in PERK-eIF2a-ATF4 signaling pathway after it acts on HepG2 cells. Materials and Method: HepG2 cells were tested with different concentrations of isoquercitrin. MTT assay was employed to detect the cell proliferation. Expressions of GRP78, PERK, elF2a, ATF4 and CHOP mRNA and protein were measured by qRT-PCR and Western blot. Results: Cell experiments showed that isoquercitrin inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner. Compared with the control group, the expressions of GRP78, PERK, elF2a, ATF4, CHOP mRNA and the expressions of GRP78, p-PERK, elF2a, ATF4, CHOP protein were significantly increased after isoquercitrin treatment, and the expressions of PERK protein decreased significantly. Conclusion: The present study verified that isoquercitrin induce the apoptosis of HepG2 cells, and its mechanism may be related to the intervention of related factors in PERK-eIF2a-ATF4 signaling pathway.
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DOI：
10.1007/978-3-319-13614-1_23
发表时间：
2012-12
期刊：
Radiopaedia.org
影响因子：
--
作者：
Andrew Murphy;Derek Smith
通讯作者：
Andrew Murphy;Derek Smith
DOI：
10.1007/s00018-015-2052-6
发表时间：
2016-01
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Schwarz DS;Blower MD
通讯作者：
Blower MD
影响因子：
5.6
作者：
Zhou R;Ma Y;Tao Z;Qiu S;Gong Z;Tao L;Zhu Y
通讯作者：
Zhu Y
影响因子：
4
作者：
Goldberg, MJ
通讯作者：
Goldberg, MJ
影响因子：
4.2
作者：
Quan Chen;Ping Li;Yong Xu;Yang Li;B. Tang
通讯作者：
Quan Chen;Ping Li;Yong Xu;Yang Li;B. Tang