A Temporal Examination of Cytoplasmic Ca(2 +) Levels, Sarcoplasmic Reticulum Ca(2 +) Levels, and Ca(2 +) -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels.
A Temporal Examination of Cytoplasmic Ca(2 +) Levels, Sarcoplasmic Reticulum Ca(2 +) Levels, and Ca(2 +) -Handling-Related Proteins in Different Skeletal Muscles of Hibernating Daurian Ground Squirrels.
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冬眠达乌尔地松鼠不同骨骼肌中细胞质 Ca2 水平、肌浆网 Ca2 水平和 Ca2 处理相关蛋白的时间检查
DOI：
10.3389/fphys.2020.562080
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发表时间：
2020
影响因子：
4
通讯作者：
Gao YF
中科院分区：
文献类型：
--
作者：
Wang Z;Zhang J;Ma XF;Chang H;Peng X;Xu SH;Wang HP;Gao YF
为探讨肌浆网（SR）维持细胞内钙稳态的可能机制，我们研究了慢收缩肌细胞内钙、SR钙和钙处理蛋白的变化（比目鱼肌，SOL），快缩肌（趾长伸肌，EDL）和混合肌（腓肠肌，GAS）在冬眠的达乌尔黄鼠（Spermophilus dauricus）的不同阶段。结果表明，在晚眠（LT）和间歇唤醒（IBA）时，骨骼肌纤维胞浆Ca ~（2+）水平升高，肌浆网Ca ~（2+）水平降低，但在动物从晚眠（ET）唤醒和再进入晚眠（ET）时，两者均恢复到夏季活动水平，表明细胞内Ca ~（2+）在冬眠期间是动态变化的。LT、IBA和ET组RyR 1蛋白表达增加，而LT和ET组GAS肌钙螯合蛋白1（CSQ 1）和RyR 1共定位减少，这可能增加了RyR 1通道介导的Ca 2+释放的可能性。此外，钙泵（SR Ca 2 +-ATP酶1，SERCA 1）蛋白表达在LT、IBA和ET组中增加，并且SERCA活性的信号通路相关因子[即，β-肾上腺素能受体2蛋白表达（在GAS中）、受磷蛋白磷酸化水平（在GAS中）和钙调蛋白激酶2（在SOL中）均增加，表明这些因素可能参与了不同组中SERCA 1活性的上调。Ca ~（2+）结合蛋白CSQ 1和钙调素（CaM）的蛋白表达增加表明LT、IBA、ET和POST组细胞内游离Ca ~（2+）结合能力也增加。简而言之，细胞质和SR Ca 2+浓度，SR RyR 1和SERCA 1蛋白表达水平，以及主要RyR 1和SERCA 1信号通路相关因子的变化在代谢功能高度抑制的休眠阶段出乎意料地活跃。
To explore the possible mechanism of the sarcoplasmic reticulum (SR) in the maintenance of cytoplasmic calcium (Ca2+) homeostasis, we studied changes in cytoplasmic Ca2+, SR Ca2+, and Ca2+-handling proteins of slow-twitch muscle (soleus, SOL), fast-twitch muscle (extensor digitorum longus, EDL), and mixed muscle (gastrocnemius, GAS) in different stages in hibernating Daurian ground squirrels (Spermophilus dauricus). Results showed that the level of cytoplasmic Ca2+ increased and SR Ca2+ decreased in skeletal muscle fiber during late torpor (LT) and inter-bout arousal (IBA), but both returned to summer active levels when the animals aroused from and re-entered into torpor (early torpor, ET), suggesting that intracellular Ca2+ is dynamic during hibernation. The protein expression of ryanodine receptor1 (RyR1) increased in the LT, IBA, and ET groups, whereas the co-localization of calsequestrin1 (CSQ1) and RyR1 in GAS muscle decreased in the LT and ET groups, which may increase the possibility of RyR1 channel-mediated Ca2+ release. Furthermore, calcium pump (SR Ca2+-ATPase 1, SERCA1) protein expression increased in the LT, IBA, and ET groups, and the signaling pathway-related factors of SERCA activity [i.e., β-adrenergic receptor2 protein expression (in GAS), phosphorylation levels of phospholamban (in GAS), and calmodulin kinase2 (in SOL)] all increased, suggesting that these factors may be involved in the up-regulation of SERCA1 activity in different groups. The increased protein expression of Ca2+-binding proteins CSQ1 and calmodulin (CaM) indicated that intracellular free Ca2+-binding ability also increased in the LT, IBA, ET, and POST groups. In brief, changes in cytoplasmic and SR Ca2+ concentrations, SR RyR1 and SERCA1 protein expression levels, and major RyR1 and SERCA1 signaling pathway-related factors were unexpectedly active in the torpor stage when metabolic functions were highly inhibited.
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影响因子：
4.6
作者：
Guo Q;Mi X;Sun X;Li X;Fu W;Xu S;Wang Q;Arfat Y;Wang H;Chang H;Gao Y
通讯作者：
Gao Y
显着保持 Ca2 稳态和抑制细胞凋亡有助于冬眠达乌尔地松鼠的抗肌肉萎缩作用
影响因子：
4.6
作者：
Fu W;Hu H;Dang K;Chang H;Du B;Wu X;Gao Y
通讯作者：
Gao Y
影响因子：
2
作者：
Lee, Young-Seon;Keener, James P.
通讯作者：
Keener, James P.
影响因子：
3.7
作者：
Lagache, Thibault;Sauvonnet, Nathalie;Olivo-Marin, Jean-Christophe
通讯作者：
Olivo-Marin, Jean-Christophe
机械卸载引起的肌肉萎缩：机制和潜在对策
影响因子：
4
作者：
Gao Y;Arfat Y;Wang H;Goswami N
通讯作者：
Goswami N