Insulin-like growth factor-1 signaling regulates miRNA expression in MCF-7 breast cancer cell line.
Insulin-like growth factor-1 signaling regulates miRNA expression in MCF-7 breast cancer cell line.
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DOI：
10.1371/journal.pone.0049067
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Burow ME
中科院分区：
文献类型：
--
作者：
Martin EC;Bratton MR;Zhu Y;Rhodes LV;Tilghman SL;Collins-Burow BM;Burow ME
在乳腺癌中，胰岛素样生长因子1 （IGF-1）水平升高可作为丝裂原，通过调控MAPK和AKT信号通路促进肿瘤发生。通过这两种途径的信号通路允许IGF-1采用有利于增殖和细胞存活的机制。在这里，我们展示了先前描述的肿瘤抑制和致癌microRNAs （miRNAs）的一个子集，它们受IGF-1信号的直接调节。此外，我们发现在IGF-1刺激之前选择性抑制MAPK或AKT通路可以阻止先前描述的具有家族和集群特异性的肿瘤抑制mirna的表达。在这里，我们首次定义了雌激素受体阳性MCF-7乳腺癌细胞系中受IGF-1信号直接调控的特异性mirna，并证明了激酶信号作为一小部分microrna的表达调节剂。综上所述，这些数据为乳腺癌中控制IGF-1信号传导的机制提供了新的见解。
In breast carcinomas, increased levels of insulin-like growth factor 1 (IGF-1) can act as a mitogen to augment tumorigenesis through the regulation of MAPK and AKT signaling pathways. Signaling through these two pathways allows IGF-1 to employ mechanisms that favor proliferation and cellular survival. Here we demonstrate a subset of previously described tumor suppressor and oncogenic microRNAs (miRNAs) that are under the direct regulation of IGF-1 signaling. Additionally, we show that the selective inhibition of either the MAPK or AKT pathways prior to IGF-1 stimulation prevents the expression of previously described tumor suppressor miRNAs that are family and cluster specific. Here we have defined, for the first time, specific miRNAs under the direct regulation of IGF-1 signaling in the estrogen receptor positive MCF-7 breast cancer cell line and demonstrate kinase signaling as a modulator of expression for a small subset of microRNAs. Taken together, these data give new insights into mechanisms governing IGF-1 signaling in breast cancer.
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影响因子：
7.3
作者：
Iliopoulos D;Polytarchou C;Hatziapostolou M;Kottakis F;Maroulakou IG;Struhl K;Tsichlis PN
通讯作者：
Tsichlis PN
影响因子：
3.9
作者：
Sachdev, D;Yee, D
通讯作者：
Yee, D
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11.2
作者：
Elyakim, Eran;Sitbon, Einat;Yerushalmi, Noga
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14.9
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Bhat-Nakshatri P;Wang G;Collins NR;Thomson MJ;Geistlinger TR;Carroll JS;Brown M;Hammond S;Srour EF;Liu Y;Nakshatri H
通讯作者：
Nakshatri H
影响因子：
4.7
作者：
Cittelly DM;Das PM;Salvo VA;Fonseca JP;Burow ME;Jones FE
通讯作者：
Jones FE