Development of a Method for the Determination of Acyl-CoA Compounds by Liquid Chromatography Mass Spectrometry to Probe the Metabolism of Fatty Acids.
Development of a Method for the Determination of Acyl-CoA Compounds by Liquid Chromatography Mass Spectrometry to Probe the Metabolism of Fatty Acids.
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DOI：
10.1021/acs.analchem.6b03623
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发表时间：
2017-01-03
影响因子：
7.4
通讯作者：
Bartlett MG
中科院分区：
文献类型：
--
作者：
Yang X;Ma Y;Li N;Cai H;Bartlett MG
Acyl-Coenzyme As (acyl-CoAs) are a group of activated fatty acid molecules participating in multiple cellular processes including lipid synthesis, oxidative metabolism of fatty acids to produce ATP, transcriptional regulation and protein posttranslational modification. Quantification of cellular acyl-CoAs is challenging due to their instability in aqueous solutions and lack of blank matrices. Here we demonstrate an LC-MS/MS analytical method which allows for absolute quantitation with broad coverage of cellular acyl-CoAs. This assay was applied to profile endogenous acyl-CoAs under the challenge of a variety of dietary fatty acids in prostate and hepatic cells. Additionally, this approach allowed for detection of multiple fatty acid metabolic processes including the biogenesis of acyl-CoAs, and their elongation, degradation, and desaturation. Hierarchical clustering in the remodeling of acyl-CoA profiles revealed a fatty acid-specific pattern across all tested cell lines, which provides a valuable reference for making predictions in other cell models. Individual acyl-CoAs were identified which were altered differentially by exogenous fatty acids in divergent tumorigenicity states of cells. These findings demonstrate the power of acyl-CoA profiling toward understanding the mechanisms for the progression of tumors or other diseases in response to fatty acids.
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影响因子：
64.5
作者：
Nomura DK;Long JZ;Niessen S;Hoover HS;Ng SW;Cravatt BF
通讯作者：
Cravatt BF
DOI：
10.1016/j.jchromb.2004.05.012
发表时间：
2004-09-05
影响因子：
3
作者：
Mauriala, T;Herzig, KH;Auriola, S
通讯作者：
Auriola, S
影响因子：
8.9
作者：
Grevengoed TJ;Klett EL;Coleman RA
通讯作者：
Coleman RA
影响因子：
4.6
作者：
Chow A;Zhou W;Liu L;Fong MY;Champer J;Van Haute D;Chin AR;Ren X;Gugiu BG;Meng Z;Huang W;Ngo V;Kortylewski M;Wang SE
通讯作者：
Wang SE
影响因子：
--
作者：
Sánchez-Martínez R;Cruz-Gil S;Gómez de Cedrón M;Álvarez-Fernández M;Vargas T;Molina S;García B;Herranz J;Moreno-Rubio J;Reglero G;Pérez-Moreno M;Feliu J;Malumbres M;Ramírez de Molina A
通讯作者：
Ramírez de Molina A