Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats.
Transplantation of cryopreserved human umbilical cord blood-derived endothelial progenitor cells induces recovery of carotid artery injury in nude rats.
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冻存人脐带血内皮祖细胞移植诱导裸鼠颈动脉损伤恢复
DOI：
10.1186/s13287-015-0022-4
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发表时间：
2015-04-17
影响因子：
7.5
通讯作者：
Zhao X
中科院分区：
文献类型：
--
作者：
Yin Y;Liu H;Wang F;Li L;Deng M;Huang L;Zhao X
内皮祖细胞（EPCs）移植可恢复内皮功能障碍和初始剥落患者的内皮功能。本研究旨在探讨人脐带血（UCB）源性EPC输注对裸鼠颈动脉损伤的修复作用。方法将体外冷冻保存的人UCB和心血管疾病患者及健康志愿者外周血的单核细胞（MNCs）在条件培养基中培养。研究EPCs在体外的迁移、增殖、粘附、存活能力以及旁分泌细胞因子的释放。还在体内评估了EPC归巢、诱导再内皮化以及对新生内膜形成的影响。结果与健康志愿者PB-EPCs （HPB-EPCs）和低温保存的UCB-EPCs相比，患者源性PB-EPCs （PPB-EPCs）的迁移、增殖、粘附和存活能力均有所下降。然而，血管内皮生长因子（VEGF）和基质细胞衍生因子1 （SDF-1）的释放在三组之间没有差异。移植2周后，通过细胞追踪发现损伤区标记的UCB-EPCs和HPB-EPCs多于PPB-EPCs。与生理盐水对照组相比，给予PPB-EPCs、HPB-EPCs和UCB-EPCs可增强再内皮化并抑制新内膜形成。但UCB-EPC和HPB-EPC输注比ppb - epc输注有更大的改善。结论体外保存的UCB-MNCs来源的EPCs和HPB-EPCs对细胞因子和血管损伤的反应优于PPB-EPCs。因此，低温保存和递送自体UCB-EPCs或HPB-EPCs可能是具有多种心血管危险因素的患者的一种有前途的血管保护方法。
IntroductionTransplantation of endothelial progenitor cells (EPCs) restores endothelial function in patients with endothelial dysfunction and initial denudation. The goal of the present study was to determine the effect of cryopreserved human umbilical cord blood (UCB)-derived EPC infusion on the repair of carotid artery injury in nude rats.MethodsMononuclear cells (MNCs) from human cryopreserved UCB and peripheral blood (PB) of patients with cardiovascular diseases and healthy volunteers were cultured in a conditioned medium. The in vitro migration, proliferation, adhesion, and survival capacities, as well as paracrine cytokine release of EPCs were investigated. EPC homing, induced reendothelialization, and the effect on neointima formation were also assessed in vivo.ResultsPatient-derived PB EPCs (PPB-EPCs) displayed decreased migration, proliferation, adhesion, and survival capabilities as compared to PB-EPCs from healthy volunteers (HPB-EPCs) and cryopreserved UCB-EPCs. However, there was no difference in the release of vascular endothelial growth factor (VEGF) and stromal cell derived factor 1 (SDF-1) between the three groups. Two weeks after transplantation, more labeled UCB-EPCs and HPB-EPCs than PPB-EPCs were found by cell tracking in the injury zone. Administration of PPB-EPCs, HPB-EPCs, and UCB-EPCs enhanced reendothelialization and inhibited neointima formation compared to the saline control. However, UCB-EPC and HPB-EPC infusion showed a greater improvement than PPB-EPCs.ConclusionsCryopreserved UCB-MNCs derived EPCs and HPB-EPCs show better responses to cytokines and vascular injury than PPB-EPCs. Thus, cryopreservation and delivery of cryopreserved autogenous UCB-EPCs or HPB-EPCs may be a promising vasculoprotective approach for patients with multiple cardiovascular risk factors.
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影响因子：
56.9
作者：
Asahara, T;Murohara, T;Isner, JM
通讯作者：
Isner, JM
影响因子：
24
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作者：
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