A comparative study of Toxoplasma gondii seroprevalence in three healthy Chinese populations detected using native and recombinant antigens.
A comparative study of Toxoplasma gondii seroprevalence in three healthy Chinese populations detected using native and recombinant antigens.
复制标题
DOI：
10.1186/1756-3305-6-241
复制
发表时间：
2013-08-20
影响因子：
3.2
通讯作者：
Jiang N
中科院分区：
文献类型：
--
作者：
Sun X;Lu H;Jia B;Chang Z;Peng S;Yin J;Chen Q;Jiang N
弓形虫病是最常见的人畜共患寄生虫病之一。弓形虫在人类中的血清阳性率在世界范围内差异很大。弓形虫特异性抗体的检测已成为流行病学调查和临床诊断的金标准方法。遗传调查表明，该寄生虫的不同基因组类型或变异体广泛分布在不同地区流行。因此，需要广泛评估使用来自单一基因组类型寄生虫的抗原用于诊断和流行病学目的的可靠性。用纯化的I型和II型弓形虫天然抗原和重组抗原，系统检测了中国地区880名临床健康人的弓形虫感染情况。用商品弓形虫病诊断试剂盒和免疫印迹试验进一步证实血清中弓形虫特异性的Ig G和Ig M。用天然I型和II型粗制抗原检测弓形虫特异性免疫球蛋白的血清流行率分别为12.2%和11.3%。而当结合重组速殖子和慢殖子抗原的结果时，总的患病率超过20%。不同个体对寄生虫抗原的免疫识别能力存在明显差异。抗-T抗体的普遍流行。研究人群中的弓形虫抗体可能比以前报道的要高得多。这些数据还表明，中国弓形虫分离株之间存在着更多的遗传多样性。此外，重组抗原与清晰的免疫识别相结合将能够产生比用弓形虫速殖子粗抗原获得的更敏感的诊断结果。
Toxoplasmosis is one of the most common parasitic zoonoses. The seroprevalence of Toxoplasma gondii infection in humans varies widely worldwide. Detection of Toxoplasma-specific antibodies has been a gold standard method for both epidemiological investigation and clinical diagnosis. Genetic investigation indicated that there is a wide distribution of different genome types or variants of the parasite prevalent in different areas. Thus the reliability of using antigens from parasites of a single genome type for diagnosis and epidemiology purposes needs to be extensively evaluated. In this study, the prevalence of T. gondii infection among 880 clinically healthy individuals in China was systematically tested using crude soluble native antigens and purified recombinant antigens of type I and II T. gondii. The T. gondii-specific IgG and IgM in the sera was further confirmed using commercial Toxoplasmosis Diagnosis Kits and Western blot assays. The sero-prevalence of T. gondii-specific IgG detected with crude native Type I and type II antigens was 12.2% and 11.3% respectively. Whereas the overall prevalence was more than 20% when combined with the results obtained with recombinant tachyzoite and bradyzoite antigens. There was an obvious variation in immune-recognition of parasite antigens among the individuals studied. The general prevalence of anti-T. gondii IgG in the study population was likely much higher than previously reported. The data also suggested that there is more genetic diversity among the T. gondii isolates in China. Further, combination of recombinant antigens with clear immuno-recognition will be able to generate more sensitive diagnostic results than those obtained with crude antigens of T. gondii tachyzoites.
登录
查看更多内容
影响因子：
3.4
作者：
BALSARI, A;POLI, G;ROLLERI, E
通讯作者：
ROLLERI, E
埃塞俄比亚西北部巴希尔·达（Bahir Dar）感染HIV和未感染个体的毒质量的血清阳性和危险因素。
影响因子：
3.2
作者：
Walle F;Kebede N;Tsegaye A;Kassa T
通讯作者：
Kassa T
影响因子：
3.7
作者：
Amerizadeh A;Khoo BY;Teh AY;Golkar M;Abdul Karim IZ;Osman S;Yunus MH;Noordin R
通讯作者：
Noordin R
影响因子：
6.6
作者：
Hinze-Selch, Dunja;Daeubener, Walter;Wilms, Sibylle
通讯作者：
Wilms, Sibylle
DOI：
10.1590/s0036-46652003000400011
发表时间：
2003-08-01
期刊：
Revista do Instituto de Medicina Tropical de São Paulo
影响因子：
--
作者：
Coêlho, Raquel A.L.;Kobayashi, Masashi;Carvalho Jr., Luiz B.
通讯作者：
Carvalho Jr., Luiz B.