Detection of human papillomavirus in branchial cleft cysts.
Detection of human papillomavirus in branchial cleft cysts.
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DOI：
10.3892/ol.2018.8827
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发表时间：
2018-08
期刊：
影响因子：
2.9
通讯作者：
Suzuki M
中科院分区：
文献类型：
--
作者：
Ikegami T;Uehara T;Deng Z;Kondo S;Maeda H;Kiyuna A;Agena S;Hirakawa H;Yamashita Y;Ganaha A;Suzuki M
据报道，鳃裂囊肿中存在高危人乳头瘤病毒 (HPV) DNA，但需要进一步的信息来阐明 HPV 感染在鳃裂囊肿中的作用。采用聚合酶链式反应 (PCR)、定量 PCR、使用 HPV DNA 探针的原位杂交 (ISH) 和 p16INK4a 免疫组织化学分析，研究了 6 名鳃裂囊肿患者样本中 HPV 的存在、病毒载量和身体状态。在所分析的 6 个鳃裂囊肿中，有 4 个被鉴定出高危型 HPV-16 DNA。在 HPV 阳性鳃裂囊肿中，有 3 个表现出 HPV 混合型整合。 ISH分析显示HPV DNA分布于囊壁基底层至颗粒层，融合患者同一层的细胞核和细胞质中p16INK4a弱表达。 ISH 显示，一名游离型感染患者的囊壁中存在 HPV DNA，但不表达 p16INK4a。两名没有 HPV 感染证据的患者在鳃裂囊肿的浅表囊肿衬里细胞中表现出弱的 p16INK4a 表达。这些结果表明，高危型 HPV 类型的感染可能在鳃裂囊肿中很常见。此外，p16INK4a 并不是鳃裂囊肿中 HPV 感染的可靠替代标记。
High-risk human papillomavirus (HPV) DNA has been reported to be present in branchial cleft cysts, but further information is required to clarify the role of HPV infection in branchial cleft cysts. The presence of HPV, the viral load and the physical statuses in samples from six patients with branchial cleft cysts were investigated using the polymerase chain reaction (PCR), quantitative PCR, in situ hybridization (ISH) using HPV DNA probes and p16INK4a immunohistochemical analysis. High-risk type HPV-16 DNA was identified in four of the six branchial cleft cysts analyzed. Of the HPV-positive branchial cleft cysts, three exhibited mixed-type integration of HPV. HPV DNA was distributed among the basal-to-granular layers of the cystic wall in ISH analysis, and p16INK4a was weakly expressed in the nuclei and cytoplasm of the same layers in patients with integration. ISH revealed that one patient with episomal-type infection exhibited HPV DNA in the cyst wall and did not express p16INK4a. Two patients without evidence of HPV infection exhibited weak p16INK4a expression in the superficial cyst-lining cells of branchial cleft cysts. These results indicate that infection with high-risk HPV types may be common in branchial cleft cysts. In addition, p16INK4a is not a reliable surrogate marker for HPV infection in branchial cleft cysts.
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DOI：
10.1016/0002-9378(92)91825-u
发表时间：
1992-01-01
影响因子：
9.8
作者：
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通讯作者：
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作者：
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