Effects of magnolol and honokiol on adhesion, yeast-hyphal transition, and formation of biofilm by Candida albicans.
Effects of magnolol and honokiol on adhesion, yeast-hyphal transition, and formation of biofilm by Candida albicans.
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厚朴酚和和厚朴酚对白色念珠菌粘附、酵母菌丝转变和生物膜形成的影响
DOI：
10.1371/journal.pone.0117695
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wang D
中科院分区：
文献类型：
--
作者：
Sun L;Liao K;Wang D
背景白色念珠菌感染的第一步是与宿主细胞或植入的医疗器械黏附，然后是菌丝生长和生物被膜的形成。长期以来，酵母到菌丝的转变一直被认为是真菌毒力的关键因素。在生物被膜形成后，白色念珠菌对抗真菌药物通常不太敏感或不敏感。因此，开发能够抑制白念珠菌黏附、菌丝转化和生物被膜形成的新型抗真菌药物是非常必要的。方法采用不同的诱导培养基，研究厚朴酚和和厚朴酚对菌丝生长的影响。用2，3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-法检测其抑制作用，用激光共聚焦显微镜观察生物膜厚度和存活率。哺乳动物细胞用于黏附实验。用实时定量逆转录-聚合酶链式反应分析菌丝发育和细胞黏附相关基因。用外源性环磷酸腺苷测定厚朴酚和和厚朴酚的作用机制。以秀丽线虫为体内模型，评价厚朴酚和和厚朴酚的抗真菌活性。结果与结论厚朴酚和和厚朴酚通过Ras1-cAMP-Efg1途径抑制白念珠菌的黏附、酵母菌向菌丝的转化和生物膜的形成。此外，厚朴酚和和厚朴酚延长了感染白色念珠菌的线虫的存活时间。厚朴酚和和厚朴酚对白色念珠菌生物被膜有潜在的抑制作用。一般意义这项研究为开发新的策略以减少白色念珠菌生物被膜相关感染提供了有用的信息。
Background The first step in infection by Candida albicans is adhesion to host cells or implanted medical devices and this followed by hyphal growth and biofilm formation. Yeast-to-hyphal transition has long been identified as a key factor in fungal virulence. Following biofilm formation, C. albicans is usually less sensitive or insensitive to antifungals. Therefore, development of new antifungals with inhibitory action on adhesion, yeast-hyphal transition and biofilm formation by C. albicans is very necessary. Methods The effects of magnolol and honokiol on hypha growth were investigated using different induction media. Their inhibitory effects were determined using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5- carboxanilide assay, and biofilm thickness and viability were observed by a confocal scanning laser microscope. Mammalian cells were used in adhesion assays. Genes related to hyphae development and cell adhesions were analyzed by real-time reverse transcription-polymerase chain reaction. The exogenous cyclic adenosine monophosphate was used to determine the mechanisms of action of magnolol and honokiol. Caenorhabditis elegans was used as an in vivo model to estimate the antifungal activities of magnolol and honokiol. Results and conclusions Magnolol and honokiol inhibited adhesion, the transition from yeast to hypha, and biofilm formation by C. albicans through the Ras1-cAMP-Efg1 pathway. Moreover, magnolol and honokiol prolonged the survival of nematodes infected by C. albicans. Magnolol and honokiol have potential inhibitory effects against biofilm formation by C. albicans. General Significance This study provides useful information towards the development of new strategies to reduce the incidence of C. albicans biofilm-associated infection.
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影响因子：
3.1
作者：
Hogan, Deborah A.;Sundstrom, Paula
通讯作者：
Sundstrom, Paula
DOI：
10.1073/pnas.1305982110
发表时间：
2013-08-13
影响因子：
11.1
作者：
Fazly, Ahmed;Jain, Charu;Kaufman, Paul D.
通讯作者：
Kaufman, Paul D.
影响因子：
6.7
作者：
Breger J;Fuchs BB;Aperis G;Moy TI;Ausubel FM;Mylonakis E
通讯作者：
Mylonakis E
影响因子：
2.4
作者：
Jin, J.;Guo, N.;Yu, L.
通讯作者：
Yu, L.
影响因子：
--
作者：
de Freitas, Marcos Motta;Pereira da Silva, Cristiano Henrique;Vidigal, Guaracilei Maciel, Jr.
通讯作者：
Vidigal, Guaracilei Maciel, Jr.