Pim-1 acts as an oncogene in human salivary gland adenoid cystic carcinoma.
Pim-1 acts as an oncogene in human salivary gland adenoid cystic carcinoma.
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Pim-1 在人类唾液腺腺样囊性癌中充当癌基因。
DOI：
10.1186/s13046-014-0114-5
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发表时间：
2014-12-31
期刊：
影响因子：
--
通讯作者：
Ge MH
中科院分区：
文献类型：
--
作者：
Zhu X;Xu JJ;Hu SS;Feng JG;Jiang LH;Hou XX;Cao J;Han J;Ling ZQ;Ge MH
Pim-1（Provirus integration site for Moloney murine leukemia virus 1）属于Ser/Thr激酶家族，在肿瘤的发生发展中起着重要作用。最近的研究表明，Pim-1磷酸化RUNX 3并改变其亚细胞定位。然而，很少有研究涉及Pim-1在涎腺腺样囊性癌（ACC）的影响。本研究旨在探讨Pim-1在体外培养ACC中的作用。同时检测ACC组织中Pim-1和RUNX 3的表达。分析Pim-1/RUNX 3水平与临床参数的相关性。用Pim-1 siRNA转染SACC-83和SACC-LM细胞。分别采用实时荧光定量PCR和免疫印迹法检测Pim-1 mRNA和蛋白表达。CCK-8法检测细胞增殖;流式细胞仪检测细胞周期、凋亡和线粒体膜电位。transwell迁移实验检测Pim-1对细胞侵袭能力的影响。免疫组化法检测ACC组织中Pim-1和RUNX 3的表达。Pim-1 siRNA可降低SACC-83和SACC-LM细胞的增殖，诱导凋亡，通过细胞周期相关蛋白（Cyclin D1和CDK 4）、线粒体去极化引起细胞周期阻滞，并降低侵袭能力。Pim-1和RUNX 3水平与ACC组织中的T分期和神经浸润显著相关。本研究证实了Pim-1在ACC中的致癌作用，并提示Pim-1可能作为ACC的一个新的治疗靶点和潜在的预后标志物。
Pim-1 (Provirus integration site for Moloney murine leukemia virus 1) belongs to the Ser/Thr kinase family and plays a pivotal role in occurrence and development of oncogenesis. Recent studies have demonstrated that Pim-1 phosphorylates RUNX3 and alters its subcellular localization. However, few studies have concerned the implications of Pim-1 in the salivary gland adenoid cystic carcinoma (ACC). In this study, we aimed to clarify the function of Pim-1 in ACC in vitro. Meanwhile, we measured the levels of Pim-1 and RUNX3 in the ACC tissues. The correlations between Pim-1/RUNX3 levels and clinical parameters were also analyzed. SACC-83 and SACC-LM cells were transfected with the Pim-1 siRNA. Pim-1 mRNA and protein expression were measured using real-time PCR and immnuoblot, respectively. Cell proliferation was analyzed by CCK-8 assay. Cell cycle, apoptosis, and mitochondrial membrane potential were detected by flow cytometry. Effects of Pim-1 on cells’ invasion were evaluated by transwell migration assay. Pim-1 and RUNX3 levels in ACC tissues were examined by immunohistochemistry. Pim-1 siRNA reduces cell proliferation, induces apoptosis, causes cell cycle arrest through cell cycle related proteins (Cyclin D1 and CDK4), mitochondrial depolarization, and decreases invasive ability in SACC-83 and SACC-LM cells. Pim-1 and RUNX3 levels are significantly relevant and associated with T-stage and nerve invasion in the ACC tissues. This study demonstrates the oncogenic role of Pim-1 in ACC. The findings also suggest that Pim-1 may serve as a neoteric therapeutic target and potential prognostic marker for ACC cancer.
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高尔基体特异性蛋白PAQR3与人胃癌的进展、转移和预后密切相关
影响因子：
50.5
作者：
Ling, Z. Q.;Guo, W.;Chen, Y.
通讯作者：
Chen, Y.
DOI：
10.1186/1756-9966-32-38
发表时间：
2013-06-08
期刊：
Journal of experimental & clinical cancer research : CR
影响因子：
--
作者：
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通讯作者：
Franzese O
影响因子：
4.8
作者：
Weirauch, Ulrike;Beckmann, Nadine;Aigner, Achim
通讯作者：
Aigner, Achim
影响因子：
7.6
作者：
Dong, L.;Wang, Y. X.;Wang, C. Y.
通讯作者：
Wang, C. Y.
影响因子：
3.8
作者：
Kim, Jongchan;Roh, Meejeon;Abdulkadir, Sarki A.
通讯作者：
Abdulkadir, Sarki A.